catalog number :
MBS706439
products type :
ELISA Kit
products full name :
Sheep Alkaline phosphatase, tissue-nonspecific isozyme, ALPL ELISA Kit
products short name :
alkaline phosphatase, liver/bone/kidney
products name syn :
Sheep Alkaline phosphatase; tissue-nonspecific isozyme (ALPL) ELISA kit; AP-TNAP; FLJ40094; FLJ93059; HOPS; MGC161443; MGC167935; TNAP; TNSALP; OTTHUMP00000002972; alkaline phosphatase; tissue-nonspecific isozyme; alkaline phosphomonoesterase; glycerophosphatase; liver/bone/; alkaline phosphatase; liver/bone/kidney
other names :
alkaline phosphatase, tissue-nonspecific isozyme; Alkaline phosphatase, tissue-nonspecific isozyme; alkaline phosphatase, tissue-nonspecific isozyme; TNSALP; AP-TNAP; liver/bone/kidney isozyme; alkaline phosphatase liver/bone/kidney isozyme; alkaline phosphatase tissue non-specific isoform; alkaline phosphatase, liver/bone/kidney<; Alkaline phosphatase liver/bone/kidney isozyme
products gene name :
ALPL
other gene names :
ALPL; ALPL; TNS-AP; AP-TNAP; TNSALP
uniprot entry name :
PPBT_BOVIN
specificity :
This assay has high sensitivity and excellent specificity for detection of Sheep ALPL. No significant cross-reactivity or interference between Sheep ALPL and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, tissue homogenates. Detection Range: 15.6 mU/ml-1000 mU/ml. Detection Wavelength: 450 nm. Sensitivity: 3.9 mU/ml
other info2 :
Sample Volume: 50-100ul
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ALPL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ALPL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ALPL is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ALPL bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_789828.2
ncbi gb acc num :
NM_176858.2
ncbi mol weight :
57,193 Da
ncbi pathways :
Endochondral Ossification Pathway 920938!!Folate Biosynthesis Pathway 84258!!Folate Biosynthesis Pathway 404!!Metabolic Pathways 132940!!TNF-alpha NF-kB Signaling Pathway 920940!!Tetrahydrofolate Biosynthesis I Mammals Pathway 123013!!Tetrahydrofolate Biosynthesis II Mammals Pathway 123012