catalog number :
MBS706435
products type :
ELISA Kit
products full name :
Rat alpha-melanocyte stimulating hormone, alpha-MSH ELISA Kit
products short name :
alpha-melanocyte stimulating hormone (alpha-MSH)
products name syn :
Rat alpha-melanocyte stimulating hormone (alpha-MSH) ELISA kit; alpha-melanocyte stimulating hormone (alpha-MSH)
other names :
alpha-melanocyte-stimulating hormone, partial; Alpha-melanocyte-stimulating hormone; Alpha-melanocyte-stimulating hormone
products gene name :
alpha-MSH
uniprot entry name :
B4XH73_CAPHI
specificity :
This assay has high sensitivity and excellent specificity for detection of rat alpha-MSH. No significant cross-reactivity or interference between rat alpha-MSH and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Competitive. Detection Range: 5 pg/ml -1000 pg/ml. Sensitivity: The minimum detectable dose of rat alpha-MSH is typically less than 2.5 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest rat alpha-MSH concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for alpha-MSH and Horseradish Peroxidase (HRP) conjugated alpha-MSH. The competitive inhibition reaction is launched between with HRP labeled alpha-MSH and unlabeled alpha-MSH with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of alpha-MSH in the sample. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
ABW34336.1
ncbi mol weight :
23,164 Da
size4 :
10x96-Strip-Wells