ELISA/assay

Dog insulin-like growth factor 1, IGF1 ELISA Kit

MBS706394

48-Strip-Wells

565 USD

ELISA Kit

Dog insulin-like growth factor 1, IGF1 ELISA Kit

insulin-like growth factor 1 (somatomedin C)

Dog insulin-like growth factor 1 (IGF1) ELISA kit; IGF1A; IGFI; OTTHUMP00000195084; insulin-like growth factor 1; insulin-like growth factor 1 (somatomedin C)

insulin-like growth factor I; Insulin-like growth factor I; insulin-like growth factor I; IGF-I; somatomedin; insulin-like growth factor IB; Somatomedin

IGF1

IGF1; IGF1; IGF-IB; IGF-1; IGF-I

IGF1_RABIT

Dog

143

This assay has high sensitivity and excellent specificity for detection of dog IGF1. No significant cross-reactivity or interference between dog IGF1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 31.25 ng/ml - 2000 ng/ml. Sensitivity: The minimum detectable dose of dog IGF1 is typically less than 7.8 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

. Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IGF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IGF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IGF1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IGF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

126722801

NP_001075495.1

NM_001082026.1

Q95222

16,092 Da

Adipogenesis Pathway (198832); Aldosterone-regulated Sodium Reabsorption Pathway (130626); Aldosterone-regulated Sodium Reabsorption Pathway (130590); Androgen Receptor Signaling Pathway (198806); Apoptosis Pathway (198797); Diabetes Pathways (105902); Dilated Cardiomyopathy Pathway (121494); Dilated Cardiomyopathy Pathway (121285); Disease Pathway (530764); Endochondral Ossification Pathway (198812)

Function: The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150