catalog number :
MBS706357
products type :
ELISA Kit
products full name :
Monkey complement C5a anaphylatoxin, C5 ELISA Kit
products short name :
complement C5a anaphylatoxin
products name syn :
Monkey complement C5a anaphylatoxin (C5) ELISA kit; CPAMD4; FLJ17816; FLJ17822; MGC142298; anaphylatoxin C5a analog; complement C5a anaphylatoxin
other names :
Complement C5a anaphylatoxin; Complement C5a anaphylatoxin; complement C5a anaphylatoxin; complement component C5; complement component 5
other gene names :
C5; C5
uniprot entry name :
CO5_BOVIN
specificity :
This assay has high sensitivity and excellent specificity for detection of monkey C5. No significant cross-reactivity or interference between monkey C5 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.625 ng/ml -40 ng/ml. Sensitivity: 0.156 ng/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for C5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any C5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for C5 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of C5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi mol weight :
8,517 Da
ncbi pathways :
Activation Of C3 And C5 Pathway (1009150); Class A/1 (Rhodopsin-like Receptors) Pathway (1008554); Complement Activation, Classical Pathway (920897); Complement And Coagulation Cascades Pathway (84312); Complement And Coagulation Cascades Pathway (484); Complement Cascade Pathway (1009143); G Alpha (i) Signalling Events Pathway (1008584); GPCR Downstream Signaling Pathway (1008582); GPCR Ligand Binding Pathway (1008553); Herpes Simplex Infection Pathway (377872)
uniprot summary :
Function: Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. C5a also stimulates the locomotion of polymorphonuclear leukocytes (chemokinesis) and direct their migration toward sites of inflammation (chemotaxis).
size4 :
10x96-Strip-Wells