catalog number :
MBS706346
products type :
ELISA Kit
products full name :
Mouse coagulation factor VIIIa light chain, F8aLC ELISA Kit
products short name :
coagulation factor VIIIa light chain (F8aLC)
products name syn :
Mouse coagulation factor VIIIa light chain (F8aLC) ELISA kit; coagulation factor VIIIa light chain (F8aLC)
products gene name :
F8aLC
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse F8aLC. No significant cross-reactivity or interference between mouse F8aLC and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Competitive. Detection Range: 1.56 ng/ml -100 ng/ml. Sensitivity: The minimum detectable dose of mouse F8aLC is typically less than 0.39 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with F8aLC. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody preparation specific for F8aLC. The competitive inhibition reaction is launched between with pre-coated F8aLC and F8aLC in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of F8aLC in the samples. The color development is stopped and the intensity of the color is measured.
size4 :
10x96-Strip-Wells