Human Troponin I, slow skeletal muscle, TNNI1 ELISA Kit | MyBioSource MBS706341 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Troponin I, slow skeletal muscle, TNNI1 ELISA Kit

catalog :

MBS706341

quantity :

24-Strip-Wells (LIMIT 1)

price :

240 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS706341

products type :

ELISA Kit

products full name :

Human Troponin I, slow skeletal muscle, TNNI1 ELISA Kit

products short name :

[troponin I type 1 (skeletal, slow)]

products name syn :

[Human Troponin I; slow skeletal muscle (TNNI1) ELISA kit; DKFZp451O223; SSTNI; TNN1; OTTHUMP00000033877; OTTHUMP00000033910; slow-twitch skeletal troponin I; troponin I; skeletal; slow; troponin I type 1 (skeletal; slow)]

other names :

[troponin I, slow skeletal muscle; Troponin I, slow skeletal muscle; troponin I, slow skeletal muscle; troponin I, slow-twitch isoform; troponin I type 1 (skeletal, slow); Troponin I, slow-twitch isoform]

products gene name :

[TNNI1]

other gene names :

[TNNI1; TNNI1; TNN1; SSTNI]

uniprot entry name :

TNNI1_HUMAN

reactivity :

Human

sequence length :

187

specificity :

This assay has high sensitivity and excellent specificity for detection of human TNNI1. No significant cross-reactivity or interference between human TNNI1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, plasma. Assay Type: Sandwich. Detection Range: 0.312 ng/ml -20 ng/ml. Sensitivity: The minimum detectable dose of human TNNI1 is typically less than 0.078 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TNNI1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TNNI1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNNI1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNNI1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

56682969

ncbi acc num :

NP_003272.3

ncbi gb acc num :

NM_003281.3

uniprot acc num :

P19237

ncbi mol weight :

21,692 Da

ncbi pathways :

Muscle Contraction Pathway (106261); Striated Muscle Contraction Pathway (198903); Striated Muscle Contraction Pathway (106262)

ncbi summary :

Troponin proteins associate with tropomyosin and regulate the calcium sensitivity of the myofibril contractile apparatus of striated muscles. Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. The TnI-fast and TnI-slow genes are expressed in fast-twitch and slow-twitch skeletal muscle fibers, respectively, while the TnI-cardiac gene is expressed exclusively in cardiac muscle tissue. This gene encodes the Troponin-I-skeletal-slow-twitch protein. This gene is expressed in cardiac and skeletal muscle during early development but is restricted to slow-twitch skeletal muscle fibers in adults. The encoded protein prevents muscle contraction by inhibiting calcium-mediated conformational changes in actin-myosin complexes. [provided by RefSeq, Jul 2008]

uniprot summary :

Function: Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

size1 :

24-Strip-Wells (LIMIT 1)

price1 :

240 USD

size2 :

48-Strip-Wells

price2 :

435

size3 :

96-Strip-Wells

price3 :

625

size4 :

5x96-Strip-Wells

price4 :

2595

size5 :

10x96-Strip-Wells

price5 :

4905

more info or order :

MyBioSource product webpage