Human Cyclin-dependent kinase inhibitor 2A, isoforms 1/2/3, CDKN2A ELISA Kit | MyBioSource MBS706339 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Cyclin-dependent kinase inhibitor 2A, isoforms 1/2/3, CDKN2A ELISA Kit

catalog :

MBS706339

quantity :

24-Strip-Wells (LIMIT 1)

price :

240 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS706339

products type :

ELISA Kit

products full name :

Human Cyclin-dependent kinase inhibitor 2A, isoforms 1/2/3, CDKN2A ELISA Kit

products short name :

[cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4)]

products name syn :

[Human Cyclin-dependent kinase inhibitor 2A; isoforms 1/2/3 (CDKN2A) ELISA kit; ARF; CDK4I; CDKN2; CMM2; INK4; INK4a; MLM; MTS1; TP16; p14; p14ARF; p16; p16INK4; p16INK4a; p19; p19Arf; CDK4 inhibitor p16-INK4; OTTHUMP00000021148; cell cycle negative regulator beta; cyclin-dependen; cyclin-dependent kinase inhibitor 2A (melanoma; p16; inhibits CDK4)]

other names :

[cyclin-dependent kinase inhibitor 2A isoform p16INK4a; Cyclin-dependent kinase inhibitor 2A, isoforms 1/2/3; cyclin-dependent kinase inhibitor 2A; CDK4 inhibitor p16-INK4; multiple tumor suppressor 1; cell cycle negative regulator beta; cyclin-dependent kinase 4 inhibitor A; cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4); cyclin-dependent kinase inhibitor 2A; Cyclin-dependent kinase 4 inhibitor A; CDK4I; Multiple tumor suppressor 1; MTS-1; p16-INK4a; p16-INK4; p16INK4A]

products gene name :

[CDKN2A]

other gene names :

[CDKN2A; CDKN2A; ARF; MLM; P14; P16; P19; CMM2; INK4; MTS1; TP16; CDK4I; CDKN2; INK4A; MTS-1; P14ARF; P19ARF; P16INK4; P16INK4A; P16-INK4A; CDKN2; MTS1; CDK4I; MTS-1; p16-INK4; p16INK4A]

uniprot entry name :

CD2A1_HUMAN

reactivity :

Human

sequence length :

156

specificity :

This assay has high sensitivity and excellent specificity for detection of human CD9. No significant cross-reactivity or interference between human CD9 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates. Assay Type: Quantitative Sandwich. Detection Range: 0.156 ng/ml-10 ng/ml. Sensitivity: 0.039 ng/ml.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CD9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CD9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CD9 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CD9 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

4502749

ncbi acc num :

NP_000068.1

ncbi gb acc num :

NM_000077.4

uniprot acc num :

P42771

ncbi mol weight :

16,533 Da

ncbi pathways :

Apoptosis Pathway (198797); Apoptosis Modulation And Signaling Pathway (198822); Bladder Cancer Pathway (83115); Bladder Cancer Pathway (527); Cell Cycle Pathway (530733); Cell Cycle, Mitotic Pathway (105765); Cell Cycle Pathway (198811); Cell Cycle Pathway (83054); Cell Cycle Pathway (463); Cellular Senescence Pathway (905991)

ncbi summary :

This gene generates several transcript variants which differ in their first exons. At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene; this transcript contains an alternate open reading frame (ARF) that specifies a protein which is structurally unrelated to the products of the other variants. This ARF product functions as a stabilizer of the tumor suppressor protein p53 as it can interact with, and sequester, the E3 ubiquitin-protein ligase MDM2, a protein responsible for the degradation of p53. In spite of the structural and functional differences, the CDK inhibitor isoforms and the ARF product encoded by this gene, through the regulatory roles of CDK4 and p53 in cell cycle G1 progression, share a common functionality in cell cycle G1 control. This gene is frequently mutated or deleted in a wide variety of tumors, and is known to be an important tumor suppressor gene. [provided by RefSeq, Sep 2012]

uniprot summary :

Function: Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein.2 PublicationsManual assertion based on experiment in:Ref.11

size1 :

24-Strip-Wells (LIMIT 1)

price1 :

240 USD

size2 :

48-Strip-Wells

price2 :

495

size3 :

96-Strip-Wells

price3 :

710

size4 :

5x96-Strip-Wells

price4 :

2705

size5 :

10x96-Strip-Wells

price5 :

5145

more info or order :

MyBioSource product webpage