product summary
Loading...
company name :
MyBioSource
product type :
ELISA/assay
product name :
Human Homeobox protein engrailed-2, EN2 ELISA Kit
catalog :
MBS706332
quantity :
48-Strip-Wells
price :
510 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS706332
products type :
ELISA Kit
products full name :
Human Homeobox protein engrailed-2, EN2 ELISA Kit
products short name :
engrailed homeobox 2
products name syn :
Human Homeobox protein engrailed-2 (EN2) ELISA kit; engrailed homolog 2; engrailed-2; engrailed homeobox 2
other names :
homeobox protein engrailed-2; Homeobox protein engrailed-2; homeobox protein engrailed-2; hu-En-2; engrailed homolog 2; homeobox protein en-2; engrailed homeobox 2
products gene name :
EN2
other gene names :
EN2; EN2; Homeobox protein en-2; Hu-En-2
uniprot entry name :
HME2_HUMAN
reactivity :
Human
sequence length :
333
specificity :
This assay has high sensitivity and excellent specificity for detection of human EN2. No significant cross-reactivity or interference between human EN2 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates, cell lysates. Assay Type: Sandwich. Detection Range: 0.312 ng/ml -20 ng/ml. Sensitivity: 0.078 ng/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for EN2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EN2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EN2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EN2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi gi num :
7710121
ncbi acc num :
NP_001418.2
ncbi gb acc num :
NM_001427.3
uniprot acc num :
P19622
ncbi mol weight :
34,211 Da
ncbi summary :
Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the 'engrailed' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system. [provided by RefSeq, Jul 2008]
uniprot summary :
En-2: Genetic variations in EN2 may be associated with susceptibility to autism. Belongs to the engrailed homeobox family. Protein type: DNA-binding; Transcription factor. Chromosomal Location of Human Ortholog: 7q36. Cellular Component: membrane; nucleus. Molecular Function: sequence-specific DNA binding. Biological Process: multicellular organismal development; midbrain development; neuron development; positive regulation of transcription from RNA polymerase II promoter; hindbrain development. Disease: Autism, Susceptibility To, 10
size1 :
48-Strip-Wells
price1 :
510 USD
size2 :
96-Strip-Wells
price2 :
725
size3 :
5x96-Strip-Wells
price3 :
2565
size4 :
10x96-Strip-Wells
price4 :
4800
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

"MyBioSource offers the best products at unbeatable prices."

Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

Would you like to receive email and e-newsletter from MyBioSource about new products, special offers and events? Please click here to join our Mailing List!