catalog number :
MBS706319
products type :
ELISA Kit
products full name :
Mouse Insulin-like peptide INSL5, INSL5 ELISA Kit
products short name :
insulin-like 5
products name syn :
Mouse Insulin-like peptide INSL5 (INSL5) ELISA kit; MGC126695; MGC126697; PRO182; UNQ156; insulin-like peptide 5; insulin-like 5
other names :
insulin-like peptide INSL5 isoform 1; Insulin-like peptide INSL5; insulin-like peptide INSL5; relaxin/insulin-like protein; relaxin/insulin-like factor 2; insulin-like 5; Relaxin/insulin-like factor 2; Relaxin/insulin-like proteinCleaved into the following 2 chains:Insulin-like peptide INSL5 B chain; Insulin-like peptide INSL5 A chain
products gene name :
INSL5
other gene names :
Insl5; Insl5; RIF2; Rif; Rif2; Zins3; Insulin-like peptide 5
uniprot entry name :
INSL5_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of Mouse INSL5. No significant cross-reactivity or interference between Mouse INSL5 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, cell culture supernates and tissue homogenates. Assay Type: Sandwich. Detection Range: 3.12 pg/ml-200 pg/ml. Sensitivity: 0.78 pg/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for INSL5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any INSL5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for INSL5 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of INSL5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001277577.1
ncbi gb acc num :
NM_001290648.1
ncbi mol weight :
15,524 Da
ncbi pathways :
Class A/1 (Rhodopsin-like Receptors) Pathway (1000942); G Alpha (i) Signalling Events Pathway (1000973); GPCR Downstream Signaling Pathway (1000971); GPCR Ligand Binding Pathway (1000941); Peptide Ligand-binding Receptors Pathway (1000943); Relaxin Receptors Pathway (1000949); Signal Transduction Pathway (970540); Signaling By GPCR Pathway (971377)
uniprot summary :
Function: May have a role in gut contractility or in thymic development and regulation. Activates RXFP4 with high potency and appears to be the endogenous ligand for this receptor
size4 :
10x96-Strip-Wells