ELISA/assay

Rat Pituitary adenylate cyclase-activating polypeptide, ADCYAP1 ELISA Kit

MBS706315

96 Strip Wells

760 USD

ELISA Kit

Rat Pituitary adenylate cyclase-activating polypeptide, ADCYAP1 ELISA Kit

adenylate cyclase activating polypeptide 1 (pituitary)

Rat Pituitary adenylate cyclase-activating polypeptide (ADCYAP1) ELISA kit; MGC126852; PACAP; OTTHUMP00000162201; adenylate cyclase activating polypeptide; adenylate cyclase activating polypeptide 1 (pituitary)

pituitary adenylate cyclase-activating polypeptide; Pituitary adenylate cyclase-activating polypeptide; pituitary adenylate cyclase-activating polypeptide; pituitary adenylate cyclase activating polypeptide 1; adenylate cyclase activating polypeptide 1 (pituitary); pituitary adenylate cyclase-activating polypeptide precursor (Pacap); adenylate cyclase activating polypeptide 1; PRP-48

ADCYAP1

Adcyap1; Adcyap1; Pacap; PACAP; PACAP-27; PACAP27; PACAP-38; PACAP38

PACA_RAT

Rat

175

This assay has high sensitivity and excellent specificity for detection of Rat ADCYAP1. No significant cross-reactivity or interference between Rat ADCYAP1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm

Sample Volume: 50-100ul

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to ADCYAP1 has been pre-coated onto a microplate. Standards or samples are added to the appropriate microtiter plate wells with biotin-conjugated ADCYAP1 and incubated. A competitive inhibition reaction is launched between ADCYAP1 (Standards or samples) and Biotin-conjugated ADCYAP1 with the pre-coated antibody specific for ADCYAP1. The more amount of ADCYAP1 in samples, the less antibody bound by Biotin-conjugated ADCYAP1. Then Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The substrate solutions are added to the wells, respectively. And the color develops in opposite to the amount of ADCYAP1 in the sample. The color development is stopped and the intensity of the color is measured.

8392855

NP_058685.1

NM_016989.2

P13589

19,557 Da

Activation Of TRKA Receptors Pathway 1010097!!Class B/2 (Secretin Family Receptors) Pathway 1010189!!G Alpha (s) Signalling Events Pathway 1010194!!GPCR Downstream Signaling Pathway 1010193!!GPCR Ligand Binding Pathway 1010163!!Glucagon-type Ligand Receptors Pathway 1010191!!Insulin Secretion Pathway 777547!!NGF Signalling Via TRKA From The Plasma Membrane Pathway 1010096!!NGF-independant TRKA Activation Pathway 1010099!!Signal Transduction Pathway 1010015

regulatory protein that stimulates cell proliferation, induces differentiation, modulates neurotransmitter release, vasodilation, bronchodilation, and hormone release, and suppresses inflammatory responses [RGD, Feb 2006]

Function: Binding to its receptor activates G proteins and stimulates adenylate cyclase in pituitary cells Promotes neuron projection development through the RAPGEF2/Rap1/B-Raf/ERK pathway.1 PublicationManual assertion based on experiment in:Ref.4

96 Strip Wells

760 USD