Monkey secreted frizzled-related protein 1, SFRP1 ELISA Kit | MyBioSource MBS706312 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Monkey secreted frizzled-related protein 1, SFRP1 ELISA Kit

catalog :

MBS706312

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS706312

products type :

ELISA Kit

products full name :

Monkey secreted frizzled-related protein 1, SFRP1 ELISA Kit

products short name :

secreted frizzled-related protein 1 (SFRP1)

products name syn :

Monkey secreted frizzled-related protein 1 (SFRP1) ELISA kit; FRP; FRP-1; FRP1; FrzA; SARP2; secreted apoptosis-related protein 2 developmental protein; secreted frizzled-related protein 1 (SFRP1)

other names :

secreted frizzled-related protein 1; Secreted frizzled-related protein 1; secreted frizzled-related protein 1; SARP-2; sFRP-1; secreted apoptosis-related protein 2; secreted frizzled-related protein 1; Secreted apoptosis-related protein 2; SARP-2

products gene name :

SFRP1

other gene names :

SFRP1; SFRP1; FRP; FRP1; FrzA; FRP-1; SARP2; FRP; FRP1; SARP2; FRP-1; sFRP-1; SARP-2

uniprot entry name :

SFRP1_HUMAN

reactivity :

Monkey

sequence length :

314

specificity :

This assay has high sensitivity and excellent specificity for detection of Monkey SFRP1. No significant cross-reactivity or interference between Monkey SFRP1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma and tissue homogenates. Assay Type: Sandwich. Detection Range: 0.156 ng/ml-10 ng/ml. Sensitivity: 0.039 ng/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450nm. Sample Volume: 50-100ul. Protein Biological Process 3: Differentiation

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SFRP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SFRP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SFRP1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SFRP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

56117838

ncbi acc num :

NP_003003.3

ncbi gb acc num :

NM_003012.4

uniprot acc num :

Q8N474

ncbi mol weight :

35,386 Da

ncbi pathways :

Disease Pathway (530764); RNF Mutants Show Enhanced WNT Signaling And Proliferation Pathway (1016209); Signal Transduction Pathway (477114); Signaling By WNT In Cancer Pathway (1016191); Signaling By Wnt Pathway (106510); TCF Dependent Signaling In Response To WNT Pathway (980475); Validated Targets Of C-MYC Transcriptional Repression Pathway (169353); Wnt Signaling Pathway NetPath (198799); Wnt Signaling Pathway (83061); Wnt Signaling Pathway (471)

ncbi summary :

This gene encodes a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. Members of this family act as soluble modulators of Wnt signaling; epigenetic silencing of SFRP genes leads to deregulated activation of the Wnt-pathway which is associated with cancer. This gene may also be involved in determining the polarity of photoreceptor cells in the retina. [provided by RefSeq, Sep 2009]

uniprot summary :

Function: Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP1 decreases intracellular beta-catenin levels Has antiproliferative effects on vascular cells, in vitro and in vivo, and can induce, in vivo, an angiogenic response. In vascular cell cycle, delays the G1 phase and entry into the S phase In kidney development, inhibits tubule formation and bud growth in metanephroi Inhibits WNT1/WNT4-mediated TCF-dependent transcription.

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

810

size3 :

5x96-Strip-Wells

price3 :

2750

size4 :

10x96-Strip-Wells

price4 :

5150

more info or order :

MyBioSource product webpage