catalog number :
MBS706295
products type :
ELISA Kit
products full name :
Rat pro interleukin 1 beta, pro-IL1B ELISA Kit
products short name :
pro interleukin 1 beta (pro-IL1B)
products name syn :
Rat pro interleukin 1 beta (pro-IL1B) ELISA Kit; pro interleukin 1 beta (pro-IL1B)
products gene name :
pro IL1B
specificity :
This assay has high sensitivity and excellent specificity for detection of rat pro-IL1B. No significant cross-reactivity or interference between rat pro-IL1B and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates, cell culture supernates. Detection Range: 62.5 pg/ml -4000 pg/ml. Sensitivity: The minimum detectable dose of rat pro-IL1B is typically less than 15.6 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Assay Type: Sandwich
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for pro-IL1B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any pro-IL1B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for pro-IL1B is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of pro-IL1B bound in the initial step. The color development is stopped and the intensity of the color is measured.
size4 :
10x96-Strip-Wells