ELISA/assay

Human Neutrophil defensin 3, DEFA3 ELISA Kit

MBS706289

48-Strip-Wells

510 USD

ELISA Kit

Human Neutrophil defensin 3, DEFA3 ELISA Kit

defensin, alpha 3, neutrophil-specific

Human Neutrophil defensin 3 (DEFA3) ELISA kit; DEF3; HNP-3; HNP3; HP-3; defensin 3; neutrophil-specific; defensin; alpha 3; neutrophil peptide 3; defensin; alpha 3; neutrophil-specific

neutrophil defensin 3 preproprotein; Neutrophil defensin 3; neutrophil defensin 3; neutrophil peptide 3; defensin 3, neutrophil-specific; defensin, alpha 3, neutrophil-specific; Defensin, alpha 3; HNP-3; HP-3; HP3

DEFA3

DEFA3; DEFA3; HP3; DEF3; HNP3; HP-3; HNP-3; DEF3; HP-3; HP3; HP-2; HP2

DEF3_HUMAN

Human

94

This assay has high sensitivity and excellent specificity for detection of Human DEFA3. No significant cross-reactivity or interference between Human DEFA3 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma. Assay Type: Sandwich. Detection Range: 1.87 ng/ml-120 ng/ml. Sensitivity: 0.47 ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Immunity. Protein Biological Process 3: Antiviral defense

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for DEFA3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DEFA3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DEFA3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DEFA3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

4885179

NP_005208.1

NM_005217.3

P59666

10,245 Da

Alpha-defensins Pathway (530758); Defensins Pathway (530757); Immune System Pathway (106386); Innate Immune System Pathway (106387); Transcriptional Misregulation In Cancer Pathway (523016); Transcriptional Misregulation In Cancer Pathway (522987)

Defensins are a family of microbicidal and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. The protein encoded by this gene, defensin, alpha 3, is found in the microbicidal granules of neutrophils and likely plays a role in phagocyte-mediated host defense. Several alpha defensin genes are clustered on chromosome 8. This gene differs from defensin, alpha 1 by only one amino acid. This gene and the gene encoding defensin, alpha 1 are both subject to copy number variation. [provided by RefSeq, May 2010]

Function: Defensin 2 and defensin 3 have antibiotic, fungicide and antiviral activities. Has antimicrobial activity against Gram-negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane.4 PublicationsManual assertion based on experiment in:Ref.8

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800