ELISA/assay

Sheep adiponectin, ADIPOQ ELISA Kit

MBS706288

48-Strip-Wells

565 USD

ELISA Kit

Sheep adiponectin, ADIPOQ ELISA Kit

adiponectin, C1Q and collagen domain containing (ADIPOQ)

Sheep adiponectin (ADIPOQ) ELISA kit; adiponectin; C1Q and collagen domain containing (ADIPOQ)

adiponectin; Adiponectin; adiponectin; apM-1; adipocyte complement related 30kDa protein; 30 kDa adipocyte complement-related protein; adipocyte complement-related 30 kDa protein; adipocyte complement related protein of 30 kDa; adipose most abundant gene transcript 1 protein; adipocyte, C1q and collagen domain-containing protein; adiponectin, C1Q and collagen domain containing; 30 kDa adipocyte complement-related protein; Adipocyte complement-related 30 kDa protein; ACRP30; Adipocyte, C1q and collagen domain-containing protein; Adipose most abundant gene transcript 1 protein; apM-1

ADIPOQ

ADIPOQ; ADIPOQ; APM1; ACRP30; ACRP30; APM1; ACRP30; apM-1

ADIPO_BOVIN

Sheep

240

This assay has high sensitivity and excellent specificity for detection of Sheep ADIPOQ. No significant cross-reactivity or interference between Sheep ADIPOQ and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Assay Type: Competitive. Detection Range: 6.25 ng/ml-100 ng/ml. Sensitivity: 3.125 ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to ADIPOQ has been pre-coated onto a microplate. Standards or samples are added to the appropriate microtiter plate wells with HRP-conjugated ADIPOQ and incubated. A competitive inhibition reaction is launched between ADIPOQ (Standards or samples) and HRP-conjugated ADIPOQ with the pre-coated antibody specific for ADIPOQ. The more amount of ADIPOQ in samples, the less pre-coated antibody specific for ADIPOQ bound by HRP-conjugated ADIPOQ. Then the substrate solutions are added to the wells, respectively. And the color develops in opposite to the amount of ADIPOQ in the sample. The color development is stopped and the intensity of the color is measured.

27807433

NP_777167.1

NM_174742.2

Q3Y5Z3

26,133 Da

AMPK Signaling Pathway (989258); AMPK Signaling Pathway (992181); Adipocytokine Signaling Pathway (84332); Adipocytokine Signaling Pathway (505); Adipogenesis Pathway (920913); Developmental Biology Pathway (1009461); Non-alcoholic Fatty Liver Disease (NAFLD) Pathway (862200); Non-alcoholic Fatty Liver Disease (NAFLD) Pathway (862314); PPAR Signaling Pathway (84281); PPAR Signaling Pathway (450)

Function: Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150