Human Glutamate [NMDA] receptor subunit zeta-1, GRIN1 ELISA Kit | MyBioSource MBS706259 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Glutamate [NMDA] receptor subunit zeta-1, GRIN1 ELISA Kit

catalog :

MBS706259

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS706259

products type :

ELISA Kit

products full name :

Human Glutamate [NMDA] receptor subunit zeta-1, GRIN1 ELISA Kit

products short name :

glutamate receptor, ionotropic, N-methyl D-aspartate 1

products name syn :

Human Glutamate [NMDA] receptor subunit zeta-1 (GRIN1) ELISA kit; NMDA1; NMDAR1; NR1; N-methyl-D-aspartate receptor channel; subunit zeta-1; NMDA receptor 1; glutamate [NMDA] receptor subunit zeta 1; glutamate receptor; ionotropic; N-methyl D-aspartate 1

other names :

glutamate receptor ionotropic, NMDA 1 isoform NR1-1; Glutamate receptor ionotropic, NMDA 1; glutamate receptor ionotropic, NMDA 1; NMD-R1; glutamate [NMDA] receptor subunit zeta 1; glutamate [NMDA] receptor subunit zeta-1; N-methyl-D-aspartate receptor subunit NR1; N-methyl-D-aspartate receptor channel, subunit zeta-1; glutamate receptor, ionotropic, N-methyl D-aspartate 1; Glutamate [NMDA] receptor subunit zeta-1; N-methyl-D-aspartate receptor subunit NR1; NMD-R1

products gene name :

GRIN1

other gene names :

GRIN1; GRIN1; NR1; MRD8; GluN1; NMDA1; NMDAR1; NMDAR1; GluN1; NMD-R1

uniprot entry name :

NMDZ1_HUMAN

reactivity :

Human

sequence length :

885

specificity :

This assay has high sensitivity and excellent specificity for detection of human GRIN1. No significant cross-reactivity or interference between human GRIN1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 125 pg/ml -8000 pg/ml. Sensitivity: The minimum detectable dose of human GRIN1 is typically less than 31.25 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GRIN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GRIN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GRIN1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GRIN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

11496971

ncbi acc num :

NP_000823.4

ncbi gb acc num :

NM_000832.6

uniprot acc num :

Q05586

ncbi mol weight :

105,373 Da

ncbi pathways :

Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway (161033); Alcoholism Pathway (585563); Alcoholism Pathway (587116); Alzheimer's Disease Pathway (83097); Alzheimer's Disease Pathway (509); Alzheimers Disease Pathway (672448); Amphetamine Addiction Pathway (547607); Amphetamine Addiction Pathway (550546); Amyotrophic Lateral Sclerosis (ALS) Pathway (83099); Amyotrophic Lateral Sclerosis (ALS) Pathway (511)

ncbi summary :

The protein encoded by this gene is a critical subunit of N-methyl-D-aspartate receptors, members of the glutamate receptor channel superfamily which are heteromeric protein complexes with multiple subunits arranged to form a ligand-gated ion channel. These subunits play a key role in the plasticity of synapses, which is believed to underlie memory and learning. Cell-specific factors are thought to control expression of different isoforms, possibly contributing to the functional diversity of the subunits. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2008]

uniprot summary :

Function: NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. This protein plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. It mediates neuronal functions in glutamate neurotransmission. Is involved in the cell surface targeting of NMDA receptors

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage