ELISA/assay

Mouse coagulation factor V, FV ELISA Kit

MBS706255

48-Strip-Wells

510 USD

ELISA Kit

Mouse coagulation factor V, FV ELISA Kit

coagulation factor V, FV

Mouse coagulation factor V; FV ELISA Kit; FVL; PCCF; activated protein c cofactor; coagulation factor V; coagulation factor V jinjiang A2 domain; factor V Leiden; coagulation factor V; FV

coagulation factor V; Coagulation factor V; coagulation factor V; activated protein C cofactor; coagulation factor V; Activated protein C cofactorCleaved into the following 2 chains:Coagulation factor V heavy chain; Coagulation factor V light chain

FV

F5; F5; Cf5; Cf-5; AI173222

FA5_MOUSE

Mouse

2183

This assay has high sensitivity and excellent specificity for detection of mouse FV. No significant cross-reactivity or interference between mouse FV and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma. Assay Type: Sandwich. Detection Range: 78 ng/ml -5000 ng/ml. Sensitivity: The minimum detectable dose of mouse FV is typically less than 19.5 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FV has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FV present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FV is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FV bound in the initial step. The color development is stopped and the intensity of the color is measured.

6679731

NP_032002.1

NM_007976.2

O88783

247,230 Da

Blood Clotting Cascade Pathway (198388); Common Pathway (1001321); Complement And Coagulation Cascades Pathway (198335); Complement And Coagulation Cascades Pathway (83270); Complement And Coagulation Cascades Pathway (484); Formation Of Fibrin Clot (Clotting Cascade) Pathway (1001318); Hemostasis Pathway (1001295); Platelet Activation, Signaling And Aggregation Pathway (1001305); Platelet Degranulation Pathway (1001317); Response To Elevated Platelet Cytosolic Ca2+ Pathway (1001315)

This gene encodes a glycoprotein coagulation factor that plays a critical role in the process of blood coagulation and hemostasis. The encoded protein is activated by thrombin, to generate a heterodimer containing heavy and light chains held together by calcium ions. About half of the mice lacking the encoded protein die at an embryonic stage possible due to abnormal yolk-sac vasculature while the remaining animals succumbed to massive hemorrhage immediately after birth. A point mutation in this gene has been shown to cause disseminated intravascular thrombosis in the perinatal period, resulting in frequent deaths of newborn mice. [provided by RefSeq, Apr 2015]

Function: Central regulator of hemostasis. It serves as a critical cofactor for the prothrombinase activity of factor Xa that results in the activation of prothrombin to thrombin. Enzyme regulation: Inhibited by SERPINA5

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800