ELISA/assay

Mouse coagulation factor III, FIII ELISA Kit

MBS706253

48-Strip-Wells

510 USD

ELISA Kit

Mouse coagulation factor III, FIII ELISA Kit

coagulation factor III, FIII

Mouse coagulation factor III; FIII ELISA Kit; CD142; TF; TFA; coagulation factor III; tissue factor; coagulation factor III; F

tissue factor; Tissue factor; tissue factor; coagulation factor III; Coagulation factor III; CD_antigen: CD142

F

F3; F3; TF; Cf3; Cf-3; CD142; AA409063; Cf-3; Cf3; TF

TF_MOUSE

Mouse

294

This assay has high sensitivity and excellent specificity for detection of Mouse FIII. No significant cross-reactivity or interference between Mouse FIII and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 7.8 pg/ml-500 pg/ml. Sensitivity: 1.95 pg/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Blood Coagulation. Protein Biological Process 3: Blood coagulation

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FIII has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FIII present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FIII is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FIII bound in the initial step. The color development is stopped and the intensity of the color is measured.

170172540

NP_034301.3

NM_010171.3

P20352

32,935 Da

Complement And Coagulation Cascades Pathway (198335); Complement And Coagulation Cascades Pathway (83270); Complement And Coagulation Cascades Pathway (484); Extrinsic Pathway (1001319); Formation Of Fibrin Clot (Clotting Cascade) Pathway (1001318); Hemostasis Pathway (1001295); Macrophage Markers Pathway (672439); PodNet: Protein-protein Interactions In The Podocyte Pathway (755428); XPodNet - Protein-protein Interactions In The Podocyte Expanded By STRING Pathway (755427)

This gene encodes a membrane-bound glycoprotein that forms the primary physiological initiator of the blood coagulation process following vascular damage. The encoded protein binds to coagulation factor VIIa and the ensuing complex catalyzes the proteolytic activation of coagulation factors IX and X. Mice lacking encoded protein die in utero resulting from massive hemorrhaging in both extraembryonic and embryonic vessels. A severe deficiency of the encoded protein in mice results in impaired uterine homeostasis, shorter life spans due to spontaneous fatal hemorrhages and cardiac fibrosis. [provided by RefSeq, Aug 2015]

Function: Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800