catalog number :
MBS706250
products type :
ELISA Kit
products full name :
Canine Parathyroid hormone, PTH ELISA Kit
products short name :
Parathyroid hormone (PTH)
products name syn :
Canine Parathyroid hormone (PTH) ELISA Kit; PTH1; parathormone; parathyrin; parathyroid hormone 1; Parathyroid hormone (PTH)
other names :
parathyroid hormone; Parathyroid hormone; parathyroid hormone; parathyrin; Parathyrin
other gene names :
PTH; PTH; PTH
uniprot entry name :
PTHY_CANFA
specificity :
This assay has high sensitivity and excellent specificity for detection of Dog PTH. No significant cross-reactivity or interference between Dog PTH and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, tissue homogenates. Detection Range: 15.6 pg/ml-1000 pg/ml. Detection Wavelength: 450 nm. Sensitivity: 3.9 pg/ml
other info2 :
Sample Volume: 50-100ul
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PTH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PTH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTH is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTH bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001003302.1
ncbi gb acc num :
NM_001003302.1
ncbi mol weight :
12,957 Da
ncbi pathways :
Class B/2 (Secretin Family Receptors) Pathway 1017214!!G Alpha (s) Signalling Events Pathway 1017219!!GPCR Downstream Signaling Pathway 1017218!!GPCR Ligand Binding Pathway 1017188!!Signal Transduction Pathway 1017095!!Signaling By GPCR Pathway 1017187
uniprot summary :
Function: PTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion. Stimulates [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells