Human protein-arginine deiminase type-4, PADI4 ELISA Kit | MyBioSource MBS706202 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human protein-arginine deiminase type-4, PADI4 ELISA Kit

catalog :

MBS706202

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS706202

products type :

ELISA Kit

products full name :

Human protein-arginine deiminase type-4, PADI4 ELISA Kit

products short name :

peptidyl arginine deiminase, type IV

products name syn :

Human protein-arginine deiminase type-4 (PADI4) ELISA kit; PAD; PAD4; PADI5; PDI4; PDI5; HL-60 PAD; PADI-H protein; peptidyl arginine deiminase; type V; protein-arginine deiminase; peptidyl arginine deiminase; type IV

other names :

protein-arginine deiminase type-4; Protein-arginine deiminase type-4; protein-arginine deiminase type-4; HL-60 PAD; PADI-H protein; protein-arginine deiminase type IV; peptidyl arginine deiminase, type V; peptidyl arginine deiminase, type IV; HL-60 PAD; Peptidylarginine deiminase IV; Protein-arginine deiminase type IV

products gene name :

PADI4

other gene names :

PADI4; PADI4; PAD; PAD4; PDI4; PDI5; PADI5; PADI5; PDI5

uniprot entry name :

PADI4_HUMAN

reactivity :

Human

sequence length :

663

specificity :

This assay has high sensitivity and excellent specificity for detection of human PADI4. No significant cross-reactivity or interference between human PADI4 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, urine, cell culture supernates. Assay Type: Sandwich. Detection Range: 0.156 ng/ml -10 ng/ml. Sensitivity: 0.039 ng/ml.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PADI4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PADI4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PADI4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PADI4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

216548487

ncbi acc num :

NP_036519.2

ncbi gb acc num :

NM_012387.2

uniprot acc num :

Q9UM07

ncbi mol weight :

74,079 Da

ncbi pathways :

Protein Citrullination Pathway (142167); Protein Citrullination Pathway (138213)

ncbi summary :

This gene is a member of a gene family which encodes enzymes responsible for the conversion of arginine residues to citrulline residues. This gene may play a role in granulocyte and macrophage development leading to inflammation and immune response. [provided by RefSeq, Jul 2008]

uniprot summary :

Function: Catalyzes the citrullination/deimination of arginine residues of proteins such as histones, thereby playing a key role in histone code and regulation of stem cell maintenance. Citrullinates histone H1 at 'Arg-54' (to form H1R54ci), histone H3 at 'Arg-2', 'Arg-8', 'Arg-17' and/or 'Arg-26' (to form H3R2ci, H3R8ci, H3R17ci, H3R26ci, respectively) and histone H4 at 'Arg-3' (to form H4R3ci). Acts as a key regulator of stem cell maintenance by mediating citrullination of histone H1: citrullination of 'Arg-54' of histone H1 (H1R54ci) results in H1 displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance. Promotes profound chromatin decondensation during the innate immune response to infection in neutrophils by mediating formation of H1R54ci. Citrullination of histone H3 prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription. Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1.6 PublicationsManual assertion based on experiment in:Ref.8. Catalytic activity: Protein L-arginine + H2O = protein L-citrulline + NH3.2 PublicationsManual assertion based on experiment in:Ref.15. Cofactor: Binds 5 calcium ions per subunit.3 PublicationsManual assertion based on experiment in:Ref.10. Enzyme regulation: Strongly Inhibited by F-amidine and N-alpha-benzoyl-N5-(2-chloro-1-iminoethyl)-L-ornithine amide (Cl-amidine). These inhibitors are however not specific to PADI4 and also inhibit other members of the family (1 PublicationRef.16

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage