catalog number :
MBS706196
products type :
ELISA Kit
products full name :
Horse Interleukin 1beta, IL-1beta ELISA Kit
products short name :
[Interleukin 1beta (IL-1beta)]
products name syn :
[Horse Interleukin 1beta (IL-1beta) ELISA Kit; IL-1; IL1-BETA; IL1F2; catabolin; preinterleukin 1 beta; pro-interleukin-1-beta; Interleukin 1beta (IL-1beta)]
other names :
[interleukin-1 beta; Interleukin-1 beta; interleukin-1 beta; IL-1 beta]
products gene name :
[IL-1beta]
other gene names :
[IL1B; IL1B; IL-1B; IL-1 beta]
uniprot entry name :
IL1B_HORSE
specificity :
This assay has high sensitivity and excellent specificity for detection of horse IL-1beta. No significant cross-reactivity or interference between horse IL-1beta and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 15.6 pg/ml -1000 pg/ml. Sensitivity: 3.9 pg/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL-1beta has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-1beta present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-1beta is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-1beta bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001075995.1
ncbi gb acc num :
NM_001082526.1
ncbi mol weight :
30,268 Da
ncbi pathways :
African Trypanosomiasis Pathway (194408); African Trypanosomiasis Pathway (194323); Alzheimer's Disease Pathway (90050); Alzheimer's Disease Pathway (509); Amoebiasis Pathway (167381); Amoebiasis Pathway (167191); Apoptosis Pathway (90013); Apoptosis Pathway (470); Chagas Disease (American Trypanosomiasis) Pathway (147818); Chagas Disease (American Trypanosomiasis) Pathway (147795)
uniprot summary :
Function: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells
size1 :
24-Strip-Wells (LIMIT 1)
size5 :
10x96-Strip-Wells