catalog number :
MBS706180
products type :
ELISA Kit
products full name :
Rat Low Density Lipoprotein cholesterol, LDL-C ELISA Kit
products short name :
Low Density Lipoprotein cholesterol (LDL-C)
products name syn :
Rat Low Density Lipoprotein cholesterol (LDL-C) ELISA kit; Low Density Lipoprotein cholesterol (LDL-C)
products gene name :
LDL-C
specificity :
This assay has high sensitivity and excellent specificity for detection of rat LDL-C. No significant cross-reactivity or interference between rat LDL-C and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: For the quantitative determination of rat low density lipoprotein cholesterol (LDL-C) concentrations in serum, plasma, tissue homogenates. Detection Range: 125 ng/ml -8000 ng/ml.
other info2 :
Sensitivity: The minimum detectable dose of rat LDL-C is typically less than 62.5 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest rat LDL-C concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: </b<This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with LDL-C. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody preparation specific for LDL-C. The competitive inhibition reaction is launched between with pre-coated LDL-C and LDL-C in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of LDL-C in the sample. The color development is stopped and the intensity of the color is measured.