catalog number :
MBS706179
products type :
ELISA Kit
products full name :
Bovine elastase 2, neutrophil, ELA2 ELISA Kit
products short name :
elastase 2, neutrophil (ELA2) ELISA kit
products name syn :
Bovine elastase 2; neutrophil (ELA2) ELISA kit; ELA2; GE; HLE; HNE; NE; PMN-E; SCN1; bone marrow serine protease; elastase 2; neutrophil; granulocyte-derived elastase; leukocyte elastase; medullasin; neutrophil elastase; polymorphonuclear elastase; elastase 2; neutrophil (ELA2) ELISA kit
other names :
chymotrypsin-like elastase family member 2A; Chymotrypsin-like elastase family member 2A; chymotrypsin-like elastase family member 2A; elastase 2; elastase-2; elastase 2A; elastase II; elastase-2A; chymotrypsin-like elastase family, member 2A<; Elastase II; Elastase-2; Elastase-2A
products gene name :
ELA2
other gene names :
CELA2A; CELA2A; Ela2; ELA2A; ELA2; ELA2A
uniprot entry name :
CEL2A_BOVIN
specificity :
This assay has high sensitivity and excellent specificity for detection of Bovine ELA2. No significant cross-reactivity or interference between Bovine ELA2 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma. Detection Range: 6.25 ng/ml-400 ng/ml. Detection Wavelength: 450 nm. Sensitivity: 1.56 ng/ml
other info2 :
Sample Volume: 50-100ul
products description :
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ELA2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for ELA2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain ELA2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of ELA2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ncbi acc num :
NP_777139.1
ncbi gb acc num :
NM_174714.2
ncbi mol weight :
28,857 Da
ncbi pathways :
Pancreatic Secretion Pathway 169305!!Pancreatic Secretion Pathway 169295!!Protein Digestion And Absorption Pathway 172830!!Protein Digestion And Absorption Pathway 171868
uniprot summary :
Function: Acts upon elastin. Catalytic activity: Preferential cleavage: Leu- -Xaa, Met- -Xaa and Phe- -Xaa. Hydrolyzes elastin.