ELISA/assay

Human Collagen Type VI, COLVI ELISA Kit

MBS706172

48-Strip-Wells

510 USD

ELISA Kit

Human Collagen Type VI, COLVI ELISA Kit

collagen, type VI, alpha 1

Human Collagen Type VI (COLVI) ELISA Kit; OPLL; alpha 1 (VI) chain (61 AA) ; collagen VI; alpha-1 polypeptide; collagen; type VI; alpha 1

collagen alpha-1(VI) chain; Collagen alpha-1(VI) chain; collagen alpha-1(VI) chain; collagen alpha-1(VI) chain; alpha 1 (VI) chain (61 AA); collagen VI, alpha-1 polypeptide; collagen, type VI, alpha 1

COL6A1

COL6A1; COL6A1; OPLL

CO6A1_HUMAN

Human

1028

This assay has high sensitivity and excellent specificity for detection of human COLVI. No significant cross-reactivity or interference between human COLVI and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, cell culture supernates, urine. Assay Type: Sandwich. Detection Range: 31.25 pg/ml -2000 pg/ml. Sensitivity: The minimum detectable dose of human COLVI is typically less than 7.81 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for COLVI has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any COLVI present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for COLVI is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of COLVI bound in the initial step. The color development is stopped and the intensity of the color is measured.

87196339

NP_001839.2

NM_001848.2

P12109

108,529 Da

Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway (730306); Axon Guidance Pathway (105688); Collagen Biosynthesis And Modifying Enzymes Pathway (645289); Collagen Formation Pathway (645288); Developmental Biology Pathway (477129); ECM-receptor Interaction Pathway (83068); ECM-receptor Interaction Pathway (479); Extracellular Matrix Organization Pathway (576262); Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478)

The collagens are a superfamily of proteins that play a role in maintaining the integrity of various tissues. Collagens are extracellular matrix proteins and have a triple-helical domain as their common structural element. Collagen VI is a major structural component of microfibrils. The basic structural unit of collagen VI is a heterotrimer of the alpha1(VI), alpha2(VI), and alpha3(VI) chains. The alpha2(VI) and alpha3(VI) chains are encoded by the COL6A2 and COL6A3 genes, respectively. The protein encoded by this gene is the alpha 1 subunit of type VI collagen (alpha1(VI) chain). Mutations in the genes that code for the collagen VI subunits result in the autosomal dominant disorder, Bethlem myopathy. [provided by RefSeq, Jul 2008]

Function: Collagen VI acts as a cell-binding protein.

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800