ELISA/assay

Human PC4 and SFRS1 interacting protein 1, PSIP1 ELISA Kit

MBS706164

48-Strip-Wells

565 USD

ELISA Kit

Human PC4 and SFRS1 interacting protein 1, PSIP1 ELISA Kit

PC4 and SFRS1 interacting protein 1

Human PC4 and SFRS1 interacting protein 1 (PSIP1) ELISA kit; DFS70; LEDGF; MGC74712; PAIP; PSIP2; p52; p75; transcriptional coactivator p52/p75; PC4 and SFRS1 interacting protein 1

PC4 and SFRS1-interacting protein isoform 2; PC4 and SFRS1-interacting protein; PC4 and SFRS1-interacting protein; DFS 70; CLL-associated antigen KW-7; dense fine speckles 70 kDa protein; transcriptional coactivator p52/p75; transcriptional coactivator p75/p52; lens epithelium-derived growth factor; PC4 and SFRS1 interacting protein 1; CLL-associated antigen KW-7; Dense fine speckles 70 kDa protein; DFS 70; Lens epithelium-derived growth factor; Transcriptional coactivator p75/p52

PSIP1

PSIP1; PSIP1; p52; p75; PAIP; DFS70; LEDGF; PSIP2; DFS70; LEDGF; PSIP2; DFS 70

PSIP1_HUMAN

Human

530

This assay has high sensitivity and excellent specificity for detection of human PSIP1. No significant cross-reactivity or interference between human PSIP1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 46.88 pg/ml -3000 pg/ml. Sensitivity: The minimum detectable dose of human PSIP1 is typically less than 11.72 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PSIP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PSIP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PSIP1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PSIP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

190014588

NP_001121689.1

NM_001128217.1

O75475

60,103 Da

2-LTR Circle Formation Pathway (105993); APOBEC3G Mediated Resistance To HIV-1 Infection Pathway (106016); Autointegration Results In Viral DNA Circles Pathway (105994); Disease Pathway (530764); Early Phase Of HIV Life Cycle Pathway (105985); HIV Infection Pathway (105983); HIV Life Cycle Pathway (105984); Host Interactions Of HIV Factors Pathway (106010); Integration Of Provirus Pathway (105991); Integration Of Viral DNA Into Host Genomic DNA Pathway (205240)

Function: Transcriptional coactivator involved in neuroepithelial stem cell differentiation and neurogenesis. Involved in particular in lens epithelial cell gene regulation and stress responses. May play an important role in lens epithelial to fiber cell terminal differentiation. May play a protective role during stress-induced apoptosis. Isoform 2 is a more general and stronger transcriptional coactivator. Isoform 2 may also act as an adapter to coordinate pre-mRNA splicing. Cellular cofactor for lentiviral integration.1 PublicationManual assertion based on experiment in:Ref.9

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150