ELISA/assay

Human RNA-binding protein FUS, FUS/TLS ELISA Kit

MBS706146

48-Strip-Wells

565 USD

ELISA Kit

Human RNA-binding protein FUS, FUS/TLS ELISA Kit

fusion (involved in t (12;16) in malignant liposarcoma)

Human RNA-binding protein FUS (FUS/TLS) ELISA kit; ALS6; FUS1; HNRNPP2; POMP75; TLS; 75 kDa DNA-pairing protein; RNA-binding protein FUS; fus-like protein; fusion gene in myxoid liposarcoma; heterogeneous nuclear ribonucleoprotein P2; oncogene TLS; transl; fusion (involved in t (12;16) in malignant liposarcoma)

RNA-binding protein FUS isoform 2; RNA-binding protein FUS; RNA-binding protein FUS; oncogene FUS; oncogene TLS; fus-like protein; fused in sarcoma; 75 kDa DNA-pairing protein; fusion gene in myxoid liposarcoma; translocated in liposarcoma protein; heterogeneous nuclear ribonucleoprotein P2; FUS RNA binding protein; 75 kDa DNA-pairing protein; Oncogene FUS; Oncogene TLS; POMp75; Translocated in liposarcoma protein

FUS

FUS; FUS; TLS; ALS6; ETM4; FUS1; POMP75; HNRNPP2; TLS

FUS_HUMAN

Human

525

This assay has high sensitivity and excellent specificity for detection of Human FUS. No significant cross-reactivity or interference between Human FUS and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to FUS. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for FUS and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain FUS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of FUS in the samples is then determined by comparing the O.D. of the samples to the standard curve.

283135201

NP_001164105.1

NM_001170634.1

P35637

53,426 Da

Gene Expression Pathway (105937); Processing Of Capped Intron-Containing Pre-mRNA Pathway (160950); Transcriptional Misregulation In Cancer Pathway (523016); Transcriptional Misregulation In Cancer Pathway (522987); MRNA Splicing Pathway (105951); MRNA Splicing - Major Pathway (105952); MRNA Processing Pathway (198843)

This gene encodes a multifunctional protein component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complex. The hnRNP complex is involved in pre-mRNA splicing and the export of fully processed mRNA to the cytoplasm. This protein belongs to the FET family of RNA-binding proteins which have been implicated in cellular processes that include regulation of gene expression, maintenance of genomic integrity and mRNA/microRNA processing. Alternative splicing results in multiple transcript variants. Defects in this gene result in amyotrophic lateral sclerosis type 6. [provided by RefSeq, Sep 2009]

Function: Binds both single-stranded and double-stranded DNA and promotes ATP-independent annealing of complementary single-stranded DNAs and D-loop formation in superhelical double-stranded DNA. May play a role in maintenance of genomic integrity.

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150