catalog number :
MBS706128
products type :
ELISA Kit
products full name :
Rat tight junction protein 1, ZO1 ELISA Kit
products short name :
tight junction protein 1 (ZO1)
products name syn :
Rat tight junction protein 1 (ZO1) ELISA Kit; DKFZp686M05161; MGC133289; ZO-1; tight junction protein 1; tight junction protein ZO-1; zona occludens 1; zonula occludens 1 protein; tight junction protein 1 (ZO1)
other names :
tight junction protein ZO-1; Tight junction protein ZO-1; tight junction protein ZO-1; zonula occludens 1; zona occludens protein 1; zonula occludens protein 1; Tight junction protein 1; Zona occludens protein 1; Zonula occludens protein 1
other gene names :
TJP1; TJP1; ZO1; ZO-1; ZO1
uniprot entry name :
ZO1_CANFA
specificity :
This assay has high sensitivity and excellent specificity for detection of rat ZO1. No significant cross-reactivity or interference between rat ZO1 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates, cell lysates. Assay Type: Sandwich. Detection Range: 31.25 pg/ml -2000 pg/ml. Sensitivity: 7.8 pg/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ZO1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ZO1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZO1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZO1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001003140.1
ncbi gb acc num :
NM_001003140.1
ncbi mol weight :
197,607 Da
ncbi pathways :
Adherens Junction Pathway (84120); Adherens Junction Pathway (481); Gap Junction Pathway (84122); Gap Junction Pathway (483); Salmonella Infection Pathway (375177); Salmonella Infection Pathway (375149); Tight Junction Pathway (84121); Tight Junction Pathway (482)
uniprot summary :
Function: The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions. Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells
size4 :
10x96-Strip-Wells