ELISA/assay

Bovine Epithelial growth factor, EGF ELISA Kit

MBS706122

48-Strip-Wells

435 USD

ELISA Kit

Bovine Epithelial growth factor, EGF ELISA Kit

Epithelial growth factor (EGF)

Bovine Epithelial growth factor (EGF) ELISA Kit; HOMG4; URG; beta-urogastrone; epidermal growth factor; Epithelial growth factor (EGF)

pro-epidermal growth factor isoform 2 preproprotein; Pro-epidermal growth factor; pro-epidermal growth factor; beta-urogastrone; epidermal growth factor; Urogastrone

EGF

EGF; EGF; URG; HOMG4; EGF

EGF_HUMAN

Bovine

1166

This assay has high sensitivity and excellent specificity for detection of Bovine EGF. No significant cross-reactivity or interference between Bovine EGF and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma. Assay Type: Sandwich. Detection Range: 0.156 ng/ml-10 ng/ml. Sensitivity: 0.039 ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for EGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EGF is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EGF bound in the initial step. The color development is stopped and the intensity of the color is measured.

296011013

NP_001171601.1

NM_001178130.1

P01133

133,994 Da

Adaptive Immune System Pathway (366160); Arf6 Signaling Events Pathway (138034); Bladder Cancer Pathway (83115); Bladder Cancer Pathway (527); Ceramide Signaling Pathway (138023); Constitutive PI3K/AKT Signaling In Cancer Pathway (685535); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); DAP12 Interactions Pathway (685549); DAP12 Signaling Pathway (685550)

This gene encodes a member of the epidermal growth factor superfamily. The encoded protein is synthesized as a large precursor molecule that is proteolytically cleaved to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding the high affinity cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2010]

Function: EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6.1 PublicationManual assertion based on experiment in:Ref.10

48-Strip-Wells

435 USD

96-Strip-Wells

615

5x96-Strip-Wells

2340

10x96-Strip-Wells

4370