ELISA/assay

Human Antigen KI-67, Ki-67 ELISA Kit

MBS706118

48-Strip-Wells

510 USD

ELISA Kit

Human Antigen KI-67, Ki-67 ELISA Kit

antigen identified by monoclonal antibody Ki-67

Human Antigen KI-67 (Ki-67) ELISA Kit; RP11-380J17.2; KIA; proliferation-related Ki-67 antigen; antigen identified by monoclonal antibody Ki-67

antigen KI-67 isoform 2; Antigen KI-67; antigen KI-67; proliferation-related Ki-67 antigen; protein phosphatase 1, regulatory subunit 105; antigen identified by monoclonal antibody Ki-67; marker of proliferation Ki-67

MKI67

MKI67; MKI67; KIA; MIB-; MIB-1; PPP1R105

KI67_HUMAN

Human

2896

This assay has high sensitivity and excellent specificity for detection of human Ki-67. No significant cross-reactivity or interference between human Ki-67 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.312 ng/ml-20 ng/ml. Sensitivity: 0.078 ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for Ki-67 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Ki-67 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Ki-67 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Ki-67 bound in the initial step. The color development is stopped and the intensity of the color is measured.

225543215

NP_001139438.1

NM_001145966.1

P46013

358,694 Da

This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. [provided by RefSeq, Mar 2009]

Function: Thought to be required for maintaining cell proliferation.

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800