ELISA/assay

Rat Syndecan-1/CD138, SDC1 ELISA Kit

MBS706069

48-Strip-Wells

565 USD

ELISA Kit

Rat Syndecan-1/CD138, SDC1 ELISA Kit

Syndecan-1/CD138 (SDC1)

Rat Syndecan-1/CD138 (SDC1) ELISA Kit; CD138; SDC; SYND1; syndecan; CD138 antigen; heparan sulfate proteoglycan fibroblast growth factor receptor; syndecan proteoglycan 1; Syndecan-1/CD138 (SDC1)

syndecan-1; Syndecan-1; syndecan-1; syndecan 1; CD_antigen: CD138

SDC1

Sdc1; Sdc1; HSPG; Synd1; SYNDECA; Syndecan; Synd1; SYND1

SDC1_RAT

Rat

313

This assay has high sensitivity and excellent specificity for detection of rat SDC1. No significant cross-reactivity or interference between rat SDC1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.312 ng/ml -20 ng/ml. Sensitivity: The minimum detectable dose of rat SDC1 is typically less than 0.078 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SDC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SDC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SDC1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SDC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

6981518

NP_037158.1

NM_013026.2

P26260

33,214 Da

A Tetrasaccharide Linker Sequence Is Required For GAG Synthesis Pathway (1010515); Cell Adhesion Molecules (CAMs) Pathway (83461); Cell Adhesion Molecules (CAMs) Pathway (480); Chondroitin Sulfate/dermatan Sulfate Metabolism Pathway (1010518); Chylomicron-mediated Lipid Transport Pathway (1010687); Disease Pathway (1010484); Diseases Associated With Visual Transduction Pathway (1010532); ECM-receptor Interaction Pathway (83460); ECM-receptor Interaction Pathway (479); Extracellular Matrix Organization Pathway (1009923)

mouse homolog promotes cell-cell adhesion [RGD, Feb 2006]

Function: Cell surface proteoglycan that bears both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix.

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150