product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Human cartilage leucine-rich protein, CHAD ELISA Kit
catalog :
MBS706050
quantity :
48-Strip-Wells
price :
510 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS706050
products type :
ELISA Kit
products full name :
Human cartilage leucine-rich protein, CHAD ELISA Kit
products short name :
chondroadherin
products name syn :
Human cartilage leucine-rich protein (CHAD) ELISA Kit; SLRR4A; chondroadherin proteoglycan; chondroadherin
other names :
chondroadherin; Chondroadherin; chondroadherin; chondroadherin proteoglycan; cartilage leucine-rich protein; chondroadherin; Cartilage leucine-rich protein
products gene name :
CHAD
other gene names :
CHAD; CHAD; SLRR4A; SLRR4A
uniprot entry name :
CHAD_HUMAN
reactivity :
Human
sequence length :
359
specificity :
This assay has high sensitivity and excellent specificity for detection of human CHAD. No significant cross-reactivity or interference between human CHAD and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 0.156 ng/ml -10 ng/ml. Sensitivity: The minimum detectable dose of human CHAD is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CHAD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CHAD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CHAD is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CHAD bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi gi num :
153251229
ncbi acc num :
NP_001258.2
ncbi gb acc num :
NM_001267.2
uniprot acc num :
O15335
ncbi mol weight :
40,476 Da
ncbi pathways :
ECM-receptor Interaction Pathway (83068); ECM-receptor Interaction Pathway (479); Focal Adhesion Pathway (198795); Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); PI3K-Akt Signaling Pathway (692234); PI3K-Akt Signaling Pathway (692979)
ncbi summary :
Chondroadherin is a cartilage matrix protein thought to mediate adhesion of isolated chondrocytes. The protein contains 11 leucine-rich repeats flanked by cysteine-rich regions. The chondroadherin messenger RNA is present in chondrocytes at all ages. [provided by RefSeq, Jul 2008]
uniprot summary :
Function: Promotes attachment of chondrocytes, fibroblasts, and osteoblasts. This binding is mediated (at least for chondrocytes and fibroblasts) by the integrin alpha2beta1. May play an important role in the regulation of chondrocyte growth and proliferation
size1 :
48-Strip-Wells
price1 :
510 USD
size2 :
96-Strip-Wells
price2 :
725
size3 :
5x96-Strip-Wells
price3 :
2565
size4 :
10x96-Strip-Wells
price4 :
4800
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

"MyBioSource offers the best products at unbeatable prices."

Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

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