ELISA/assay

Human Laminin beta1, LN-beta1 ELISA Kit

MBS706048

48-Strip-Wells

565 USD

ELISA Kit

Human Laminin beta1, LN-beta1 ELISA Kit

laminin, beta 1

Human Laminin beta1 (LN-beta1) ELISA Kit; CLM; MGC142015; cutis laxa with marfanoid phenotype; laminin; beta 1

laminin subunit beta-1; Laminin subunit beta-1; laminin subunit beta-1; laminin B1 chain; laminin, beta 1; Laminin B1 chain; Laminin-1 subunit beta; Laminin-10 subunit beta; Laminin-12 subunit beta; Laminin-2 subunit beta; Laminin-6 subunit beta; Laminin-8 subunit beta

LAMB1

LAMB1; LAMB1; CLM; LIS5

LAMB1_HUMAN

Human

1786

This assay has high sensitivity and excellent specificity for detection of Human LAMB1. No significant cross-reactivity or interference between Human LAMB1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 9.38 ng/ml-600 ng/ml. Sensitivity: 2.34 ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Cell Adhesion. Protein Biological Process 3: Cell adhesion

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LN-beta1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LN-beta1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LN-beta1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LN-beta1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

167614504

NP_002282.2

NM_002291.2

P07942

198,038 Da

Alpha6-Beta4 Integrin Signaling Pathway (198807); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Axon Guidance Pathway (105688); Degradation Of The Extracellular Matrix Pathway (576263); Developmental Biology Pathway (477129); ECM Proteoglycans Pathway (833812); ECM-receptor Interaction Pathway (83068); ECM-receptor Interaction Pathway (479); Extracellular Matrix Organization Pathway (576262)

Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the beta chain isoform laminin, beta 1. The beta 1 chain has 7 structurally distinct domains which it shares with other beta chain isomers. The C-terminal helical region containing domains I and II are separated by domain alpha, domains III and V contain several EGF-like repeats, and domains IV and VI have a globular conformation. Laminin, beta 1 is expressed in most tissues that produce basement membranes, and is one of the 3 chains constituting laminin 1, the first laminin isolated from Engelbreth-Holm-Swarm (EHS) tumor. A sequence in the beta 1 chain that is involved in cell attachment, chemotaxis, and binding to the laminin receptor was identified and shown to have the capacity to inhibit metastasis. [provided by RefSeq, Aug 2011]

Function: Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Involved in the organization of the laminar architecture of cerebral cortex. It is probably required for the integrity of the basement membrane/glia limitans that serves as an anchor point for the endfeet of radial glial cells and as a physical barrier to migrating neurons. Radial glial cells play a central role in cerebral cortical development, where they act both as the proliferative unit of the cerebral cortex and a scaffold for neurons migrating toward the pial surface.1 PublicationManual assertion based on experiment in:Ref.8

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150