ELISA/assay

Human Superoxide dismutase [Mn], mitochondrial, SOD2 ELISA Kit

MBS706038

48-Strip-Wells

510 USD

ELISA Kit

Human Superoxide dismutase [Mn], mitochondrial, SOD2 ELISA Kit

superoxide dismutase 2, mitochondrial

Human Superoxide dismutase [Mn]; mitochondrial (SOD2) ELISA kit; IPO-B; MNSOD; MVCD6; Mn-SOD; Mn superoxide dismutase; indophenoloxidase B; manganese-containing superoxide dismutase; mangano-superoxide dismutase; superoxide dismutase 2; mitochondrial

superoxide dismutase; Superoxide dismutase [Mn], mitochondrial; superoxide dismutase [Mn], mitochondrial; superoxide dismutase [Mn], mitochondrial; indophenoloxidase B; Mn superoxide dismutase; mangano-superoxide dismutase; manganese-containing superoxide dismutase; superoxide dismutase 2, mitochondrial

SOD2

SOD2; SOD2; IPOB; MNSOD; MVCD6

SODM_HUMAN

Human

222

This assay has high sensitivity and excellent specificity for detection of human SOD2. No significant cross-reactivity or interference between human SOD2 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates, cell lysates and cell culture supernates. Assay Type: Sandwich. Detection Range: 15.6 pg/ml-1000 pg/ml. Sensitivity: Typically less than 3.9 pg/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SOD2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SOD2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SOD2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SOD2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

67782305

NP_000627.2

NM_000636.2

P04179

24,722 Da

Cellular Responses To Stress Pathway (645258); DNA Damage Response (only ATM Dependent) Pathway (198827); Detoxification Of Reactive Oxygen Species Pathway (941400); FoxO Family Signaling Pathway (138036); FoxO Signaling Pathway (921162); Huntington's Disease Pathway (83100); Huntington's Disease Pathway (512); Oxidative Stress Pathway (198916); Peroxisome Pathway (131226); Peroxisome Pathway (131126)

This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Function: Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.1 PublicationManual assertion based on experiment in:Ref.18. Catalytic activity: 2 superoxide + 2 H+ = O2 + H2O2. Cofactor: Binds 1 manganese ion per subunit.

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800