ELISA/assay

Mouse Indian Hedgehog, IHH ELISA Kit

MBS706033

24-Strip-Wells (LIMIT 1)

240 USD

ELISA Kit

Mouse Indian Hedgehog, IHH ELISA Kit

[Indian Hedgehog (IHH)]

[Mouse Indian Hedgehog (IHH) ELISA kit; BDA1; HHG2; Indian hedgehog homolog; Indian Hedgehog (IHH)]

[indian hedgehog protein; Indian hedgehog protein; indian hedgehog protein; HHG-2; Indian hedgehog; HHG-2Cleaved into the following 2 chains:Indian hedgehog protein N-product; Indian hedgehog protein C-product]

[IHH]

[Ihh; Ihh; IHH]

IHH_MOUSE

Mouse

449

This assay has high sensitivity and excellent specificity for detection of mouse IHH. No significant cross-reactivity or interference between mouse IHH and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, plasma, tissue homogenates. Assay Type: Quantitative Sandwich. Detection Range: 3.12 pg/ml -200 pg/ml. Sensitivity: Typically less than 0.78 pg/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IHH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IHH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IHH is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IHH bound in the initial step. The color development is stopped and the intensity of the color is measured.

14149643

NP_034674.1

NM_010544.2

P97812

45,485 Da

Endochondral Ossification Pathway (198336); Hedgehog Signaling Pathway (198414); Hedgehog Signaling Pathway (83260); Hedgehog Signaling Pathway (474)

Function: Intercellular signal essential for a variety of patterning events during development. Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. Implicated in endochondral ossification: may regulate the balance between growth and ossification of the developing bones. Induces the expression of parathyroid hormone-related protein (PTHRP).

24-Strip-Wells (LIMIT 1)

240 USD

48-Strip-Wells

550

96-Strip-Wells

795

5x96-Strip-Wells

2890

10x96-Strip-Wells

5465