product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Biotin ELISA Kit
catalog :
MBS706024
quantity :
96-Strip-Wells
price :
795 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS706024
products type :
ELISA Kit
products full name :
Biotin ELISA Kit
products short name :
Biotin ELISA Kit
products name syn :
Biotin ELISA Kit; Biotin
other names :
biotin; Biotin; Biotin
products gene name :
Biotin
other gene names :
birA; SENTW_4215
uniprot entry name :
E9A979_SALET
sequence length :
320
specificity :
This assay has high sensitivity and excellent specificity for detection of biotin. No significant cross-reactivity or interference between biotin and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma from human, mouse and other species. Assay Type: Competitive. Detection Range: 12.5 pg/ml-200 pg/ml. Sensitivity: The minimum detectable dose of biotin is typically less than 4.986 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest biotin concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the direct competitive inhibition enzyme immunoassay technique. The microplate was pre-coated with Avidin. Add Biotin (Standards or samples) to the well, and then add HRP-conjugated Biotin. A competitive inhibition reaction is launched between Biotin (Standards or samples) and HRP conjugated Biotin with the Avidin. The substrate solutions are added to the wells, respectively. And the color develops in opposite to the amount of Biotin bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi gi num :
320088538
ncbi acc num :
CBY98296.1
uniprot acc num :
E9A979
ncbi mol weight :
35,396 Da
ncbi pathways :
Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)
size1 :
96-Strip-Wells
price1 :
795 USD
size2 :
5x96-Strip-Wells
price2 :
2435
size3 :
10x96-Strip-Wells
price3 :
4555
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

"MyBioSource offers the best products at unbeatable prices."

Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

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