catalog number :
MBS706003
products type :
ELISA Kit
products full name :
Mouse Homocysteine, HCY ELISA Kit
products short name :
[Homocysteine (HCY)]
products name syn :
[Mouse Homocysteine (HCY) ELISA Kit; Homocysteine (HCY)]
other names :
[Halocyanin; Halocyanin]
products gene name :
[Hcy]
uniprot entry name :
HCY_NATPH
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse Hcy. No significant cross-reactivity or interference between mouse Hcy and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, plasma and tissue homogenates. Assay Type: Sandwich. Detection Range: 4. 7 nmol/ml - 300 nmol/ml. Sensitivity: The minimum detectable dose of mouse Hcy is typically less than 1.1 nmol/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for Hcy has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Hcy present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Hcy is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Hcy bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi mol weight :
17,222 Da
ncbi pathways :
Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)
uniprot summary :
Function: Electron donor. Binds one copper ion.
size1 :
24-Strip-Wells (LIMIT 1)
size5 :
10x96-Strip-Wells