ELISA/assay

Human Heat shock protein 75 kDa, mitochondrial, TRAP1/HSP75 ELISA Kit

MBS706000

48-Strip-Wells

565 USD

ELISA Kit

Human Heat shock protein 75 kDa, mitochondrial, TRAP1/HSP75 ELISA Kit

TNF receptor-associated protein 1

Human Heat shock protein 75 kDa; mitochondrial (TRAP1/HSP75) ELISA kit; HSP75; HSP90L; heat shock protein 75; tumor necrosis factor type 1 receptor associated protein; TNF receptor-associated protein 1

heat shock protein 75 kDa, mitochondrial isoform 2; Heat shock protein 75 kDa, mitochondrial; heat shock protein 75 kDa, mitochondrial; TNFR-associated protein 1; tumor necrosis factor type 1 receptor-associated protein; TNF receptor-associated protein 1; TNFR-associated protein 1; Tumor necrosis factor type 1 receptor-associated protein; TRAP-1

TRAP1

TRAP1; TRAP1; HSP75; HSP 75; HSP90L; TRAP-1; HSP75; HSP 75; TRAP-1

TRAP1_HUMAN

Human

651

This assay has high sensitivity and excellent specificity for detection of human TRAP1. No significant cross-reactivity or interference between human TRAP1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.6 25 ng/ml -40 ng/ml. Sensitivity: The minimum detectable dose of human TRAP1 is typically less than 0.156 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TRAP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TRAP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TRAP1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TRAP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

440309857

NP_001258978.1

NM_001272049.1

Q12931

80,110 Da

TGF-beta Receptor Signaling Pathway (198774)

This gene encodes a mitochondrial chaperone protein that is member of the heat shock protein 90 (HSP90) family. The encoded protein has ATPase activity and interacts with tumor necrosis factor type I. This protein may function in regulating cellular stress responses. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013]

Function: Chaperone that expresses an ATPase activity. Involved in maintaining mitochondrial function and polarization, most likely through stabilization of mitochondrial complex I. Is a negative regulator of mitochondrial respiration able to modulate the balance between oxidative phosphorylation and aerobic glycolysis. The impact of TRAP1 on mitochondrial respiration is probably mediated by modulation of mitochondrial SRC and inhibition of SDHA.3 PublicationsManual assertion based on experiment in:Ref.12

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150