product summary
Loading...
company name :
MyBioSource
product type :
ELISA/assay
product name :
Rat Mast Cell Chymase ELISA Kit
catalog :
MBS705990
quantity :
96 Strip Wells
price :
850 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS705990
products type :
ELISA Kit
products full name :
Rat Mast Cell Chymase ELISA Kit
products short name :
Mast Cell Chymase
products name syn :
Rat Mast Cell Chymase ELISA Kit; CYH; MCT1; MGC119890; MGC119891; chymase; chymase 1 preproprotein transcript E; chymase 1 preproprotein transcript I; chymase; heart; chymase; mast cell; mast cell protease I; Mast Cell Chymase
other names :
Chymase; Chymase; Alpha-chymase
products gene name :
CMA1
other gene names :
CMA1
uniprot entry name :
CMA1_RABIT
reactivity :
Rat
sequence length :
220
specificity :
This assay has high sensitivity and excellent specificity for detection of Rat CMA1. No significant cross-reactivity or interference between Rat CMA1 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma. Detection Range: 1.56 ng/ml-100 ng/ml. Detection Wavelength: 450 nm. Sensitivity: 0.39 ng/ml
other info2 :
Sample Volume: 50-100ul
products description :
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MAST CELL CHYMASE. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for MAST CELL CHYMASE and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain MAST CELL CHYMASE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of MAST CELL CHYMASE in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ncbi gi num :
162416180
ncbi acc num :
P85202.1
uniprot acc num :
P85202
ncbi mol weight :
24,710 Da
ncbi pathways :
Focal Adhesion Pathway 83067!!Focal Adhesion Pathway 478!!MAPK Signaling Pathway 83048!!MAPK Signaling Pathway 456!!Salmonella Infection Pathway 375172!!Salmonella Infection Pathway 375149
uniprot summary :
Function: Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion By similarityManual assertion inferred from sequence similarityUniProtKB:P23946. Catalytic activity: Preferential cleavage: Phe- -Xaa > Tyr- -Xaa > Trp- -Xaa > Leu- -Xaa.By similarityManual assertion inferred from sequence similarityUniProtKB:P23946
size :
96 Strip Wells
price :
850 USD
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

"MyBioSource offers the best products at unbeatable prices."

Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

Would you like to receive email and e-newsletter from MyBioSource about new products, special offers and events? Please click here to join our Mailing List!