ELISA/assay

Human Aldose Reductase, AR ELISA Kit

MBS705979

48-Strip-Wells

565 USD

ELISA Kit

Human Aldose Reductase, AR ELISA Kit

aldo-keto reductase family 1, member B1 (aldose reductase)

Human Aldose Reductase (AR) ELISA Kit; ADR; ALDR1; ALR2; AR; MGC1804; Lii5-2 CTCL tumor antigen; aldehyde reductase 1; aldo-keto reductase family 1; member B1; aldose reductase; low Km aldose reductase; aldo-keto reductase family 1; member B1 (aldose reductase)

aldose reductase; Aldose reductase; aldose reductase; aldehyde reductase 1; low Km aldose reductase; Lii5-2 CTCL tumor antigen; aldo-keto reductase family 1 member B1; aldo-keto reductase family 1, member B1 (aldose reductase); Aldehyde reductase; Aldo-keto reductase family 1 member B1

AKR1B1

AKR1B1; AKR1B1; AR; ADR; ALR2; ALDR1; ALDR1; AR

ALDR_HUMAN

Human

316

This assay has high sensitivity and excellent specificity for detection of Human AKR1B1. No significant cross-reactivity or interference between Human AKR1B1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, urine and tissue homogenates. Assay Type: Sandwich. Detection Range: 0.312 ng/ml-20 ng/ml. Sensitivity: 0.078 ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for AR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AR is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AR bound in the initial step. The color development is stopped and the intensity of the color is measured.

4502049

NP_001619.1

NM_001628.2

P15121

35,853 Da

Fructose And Mannose Metabolism Pathway (82930); Fructose And Mannose Metabolism Pathway (291); Galactose Metabolism Pathway (82931); Galactose Metabolism Pathway (292); Glycerolipid Metabolism Pathway (82986); Glycerolipid Metabolism Pathway (361); Metabolic Pathways (132956); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); Metabolism Of Steroid Hormones And Vitamin D Pathway (106150)

This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member catalyzes the reduction of a number of aldehydes, including the aldehyde form of glucose, and is thereby implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol. Multiple pseudogenes have been identified for this gene. The nomenclature system used by the HUGO Gene Nomenclature Committee to define human aldo-keto reductase family members is known to differ from that used by the Mouse Genome Informatics database. [provided by RefSeq, Feb 2009]

Function: Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols with a broad range of catalytic efficiencies. Catalytic activity: Alditol + NAD(P)+ = aldose + NAD(P)H. Enzyme regulation: Cys-299 may regulate the kinetic and inhibition properties of the enzyme, but does not participate in catalysis.1 PublicationManual assertion based on experiment in:Ref.18

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150