ELISA/assay

Plant Ribulose-1, 5-bisphosphate carboxylase/oxygenase, RuBisCO ELISA Kit

MBS705973

96-Strip-Wells

795 USD

ELISA Kit

Plant Ribulose-1, 5-bisphosphate carboxylase/oxygenase, RuBisCO ELISA Kit

Ribulose-1, 5-bisphosphate carboxylase/oxygenase (RuBisCO)

Plant Ribulose-1; 5-bisphosphate carboxylase/oxygenase (RuBisCO) ELISA Kit; Plant Ribulose-1; 5-bisphosphate carboxylase/oxygenase (RuBisCO)

ribulose-1 (chloroplast); Ribulose bisphosphate carboxylase large chain

RuBisCO

rbcL; RuBisCO large subunit

W0UQ92_SALMI

Plant

484

This assay has high sensitivity and excellent specificity for detection of plant RuBisCO. No significant cross-reactivity or interference between plant RuBisCO and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Plant tissues. Assay Type: Competitive. Detection Range: 3.12 ug/ml -800 ug/ml. Sensitivity: 1.56 ug/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<10%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<20%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with antigen. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for RuBisCO. The competitive inhibition reaction is launched between with pre-coated RuBisCO and RuBisCO in samples with the antibody. Then add a Horseradish Peroxidase (HRP) conjugated goat-anti-rabbit IgG antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of RuBisCO in the sample. The color development is stopped and the intensity of the color is measured.

573461959

CCQ71628.1

W0UQ92

53,698 Da

Function: RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate in the photorespiration process. Both reactions occur simultaneously and in competition at the same active site UniRule annotationHAMAP-Rule:MF_01338. Catalytic activity: 2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O.UniRule annotationHAMAP-Rule:MF_01338. Cofactor: Binds 1 magnesium ion per subunit UniRule annotationHAMAP-Rule:MF_01338

96-Strip-Wells

795 USD

5x96-Strip-Wells

2435

10x96-Strip-Wells

4555