Human interleukin-1 receptor-associated kinase 4, IRAK-4 ELISA Kit | MyBioSource MBS705939 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human interleukin-1 receptor-associated kinase 4, IRAK-4 ELISA Kit

catalog :

MBS705939

quantity :

24-Strip-Wells (LIMIT 1)

price :

240 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS705939

products type :

ELISA Kit

products full name :

Human interleukin-1 receptor-associated kinase 4, IRAK-4 ELISA Kit

products short name :

[interleukin-1 receptor-associated kinase 4]

products name syn :

[Human interleukin-1 receptor-associated kinase 4 (IRAK-4) ELISA Kit; IPD1; NY-REN-64; REN64; ; interleukin-1 receptor-associated kinase 4]

other names :

[interleukin-1 receptor-associated kinase 4 isoform a; Interleukin-1 receptor-associated kinase 4; interleukin-1 receptor-associated kinase 4; renal carcinoma antigen NY-REN-64; interleukin-1 receptor-associated kinase 4; Renal carcinoma antigen NY-REN-64]

products gene name :

[IRAK4]

other gene names :

[IRAK4; IRAK4; IPD1; REN64; IRAK-4; NY-REN-64; IRAK-4]

uniprot entry name :

IRAK4_HUMAN

reactivity :

Human

sequence length :

460

specificity :

This assay has high sensitivity and excellent specificity for detection of human IRAK-4. No significant cross-reactivity or interference between human IRAK-4 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 6.25 pg/ml -400 pg/ml. Sensitivity: 1.56 pg/ml.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IRAK-4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IRAK-4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IRAK-4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IRAK-4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

166795295

ncbi acc num :

NP_001107654.1

ncbi gb acc num :

NM_001114182.2

uniprot acc num :

Q9NWZ3

ncbi mol weight :

51,530 Da

ncbi pathways :

AGE/RAGE Pathway (698754); Activated TLR4 Signalling Pathway (106400); Apoptosis Pathway (83060); Apoptosis Pathway (470); Chagas Disease (American Trypanosomiasis) Pathway (147809); Chagas Disease (American Trypanosomiasis) Pathway (147795); Cytokine Signaling In Immune System Pathway (366171); IL-1 Signaling Pathway (198908); IL1-mediated Signaling Events Pathway (137944); Immune System Pathway (106386)

ncbi summary :

This gene encodes a kinase that activates NF-kappaB in both the Toll-like receptor (TLR) and T-cell receptor (TCR) signaling pathways. The protein is essential for most innate immune responses. Mutations in this gene result in IRAK4 deficiency and recurrent invasive pneumococcal disease. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]

uniprot summary :

Function: Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation to form the Myddosome together with IRAK2. Phosphorylates initially IRAK1, thus stimulating the kinase activity and intensive autophosphorylation of IRAK1. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates NCF1 and regulates NADPH oxidase activation after LPS stimulation suggesting a similar mechanism during microbial infections.7 PublicationsManual assertion based on experiment in:Ref.1. Catalytic activity: ATP + a protein = ADP + a phosphoprotein. Cofactor: Magnesium.

size1 :

24-Strip-Wells (LIMIT 1)

price1 :

240 USD

size2 :

48-Strip-Wells

price2 :

495

size3 :

96-Strip-Wells

price3 :

710

size4 :

5x96-Strip-Wells

price4 :

2705

size5 :

10x96-Strip-Wells

price5 :

5145

more info or order :

MyBioSource product webpage