ELISA/assay

Human Cystatin-B, CSTB/CST6/STFB ELISA Kit

MBS705920

96-Strip-Wells

680 USD

ELISA Kit

Human Cystatin-B, CSTB/CST6/STFB ELISA Kit

cystatin B (stefin B)

Human Cystatin-B (CSTB/CST6/STFB) ELISA kit; CST6; EPM1; PME; STFB; CPI-B; cystatin B; liver thiol proteinase inhibitor; stefin B; Human Cystatin-B (CSTB/CST6/STFB)

cystatin-B; Cystatin-B; cystatin-B; CPI-B; liver thiol proteinase inhibitor; cystatin B (stefin B); CPI-B; Liver thiol proteinase inhibitor; Stefin-B

CSTB

CSTB; CSTB; PME; ULD; CST6; EPM1; STFB; EPM1A; CST6; STFB

CYTB_HUMAN

Human

98

This assay has high sensitivity and excellent specificity for detection of Human CSTB. No significant cross-reactivity or interference between Human CSTB and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, cell culture supernates, urine, saliva, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 0.313 ng/ml-20 ng/ml. Sensitivity: 0.233 ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: others

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for cystatin-B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any cystatin-B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for cystatin-B is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of cystatin-B bound in the initial step. The color development is stopped and the intensity of the color is measured.

4503117

NP_000091.1

NM_000100.3

P04080

11,140 Da

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and kininogens. This gene encodes a stefin that functions as an intracellular thiol protease inhibitor. The protein is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b. The protein is thought to play a role in protecting against the proteases leaking from lysosomes. Evidence indicates that mutations in this gene are responsible for the primary defects in patients with progressive myoclonic epilepsy (EPM1). [provided by RefSeq, Jul 2008]

Function: This is an intracellular thiol proteinase inhibitor. Tightly binding reversible inhibitor of cathepsins L, H and B.

96-Strip-Wells

680 USD

5x96-Strip-Wells

2140

10x96-Strip-Wells

3990