catalog number :
MBS705912
products type :
ELISA Kit
products full name :
Bovine lipopolysaccharide binding protein, LBP ELISA Kit
products short name :
lipopolysaccharide binding protein
products name syn :
Bovine lipopolysaccharide binding protein (LBP) ELISA kit; MGC22233; LPS-binding protein; lipopolysaccharide-binding protein; lipopolysaccharide binding protein
other names :
lipopolysaccharide-binding protein; Lipopolysaccharide-binding protein; lipopolysaccharide-binding protein; lipopolysaccharide binding protein<
other gene names :
LBP; LBP; LBP
uniprot entry name :
LBP_BOVIN
specificity :
This assay has high sensitivity and excellent specificity for detection of bovine LBP. No significant cross-reactivity or interference between bovine LBP and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 15.6 ng/ml-1000 ng/ml. Sensitivity: The minimum detectable dose of bovine LBP is typically less than 3.9 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LBP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LBP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LBP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LBP bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001033763.1
ncbi gb acc num :
NM_001038674.2
ncbi mol weight :
53,698 Da
ncbi pathways :
NF-kappa B Signaling Pathway (634529); Salmonella Infection Pathway (375171); Salmonella Infection Pathway (375149); Toll-like Receptor Signaling Pathway (84315); Toll-like Receptor Signaling Pathway (920909); Toll-like Receptor Signaling Pathway (487); Tuberculosis Pathway (213779); Tuberculosis Pathway (213743)
uniprot summary :
Function: Binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria, and acts as an affinity enhancer for CD14, facilitating its association with LPS. Promotes the release of cytokines in response to bacterial lipopolysaccharide
size4 :
10x96-Strip-Wells