ELISA/assay

Mouse Apolipoprotein A5, apo-A5 ELISA Kit

MBS705910

96-Strip-Wells

915 USD

ELISA Kit

Mouse Apolipoprotein A5, apo-A5 ELISA Kit

apolipoprotein A-V

Mouse Apolipoprotein A5 (apo-A5) ELISA Kit; APOAV; FLJ97995; MGC126836; MGC126838; RAP3; apolipoprotein A5; regeneration-associated protein 3; apolipoprotein A-V

apolipoprotein A-V; Apolipoprotein A-V; apolipoprotein A-V; apo-AV; apoA-V; apolipoprotein A5; regeneration-associated protein 3; apolipoprotein A-V; Apolipoprotein A5; Regeneration-associated protein 3

APOA5

Apoa5; Apoa5; RAP3; Apoav; 1300007O05Rik; Rap3; Apo-AV; ApoA-V

APOA5_MOUSE

Mouse

368

This assay has high sensitivity and excellent specificity for detection of mouse apo-A5. No significant cross-reactivity or interference between mouse apo-A5 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 15.6 pg/ml -1000 pg/ml. Sensitivity: The minimum detectable dose of mouse apo-A5 is typically less than 3.9 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for apo-A5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any apo-A5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for apo-A5 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of apo-A5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

31560003

NP_536682.2

NM_080434.3

Q8C7G5

41,262 Da

Chylomicron-mediated Lipid Transport Pathway (1001042); Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway (970709); Lipid Digestion, Mobilization, And Transport Pathway (1001038); Lipoprotein Metabolism Pathway (1001041); Metabolism Pathway (971233); Metabolism Of Lipids And Lipoproteins Pathway (970615); PPAR (Peroxisome Proliferator-activated Receptor) Signaling Pathway (522983); PPAR Signaling Pathway (83239); PPAR Signaling Pathway (694605); PPAR Signaling Pathway (450)

Function: Minor apolipoprotein mainly associated with HDL and to a lesser extent with VLDL. May also be associated with chylomicrons. Important determinant of plasma triglyceride (TG) levels by both being a potent stimulator of apo-CII lipoprotein lipase (LPL) TG hydrolysis and a inhibitor of the hepatic VLDL-TG production rate (without affecting the VLDL-apoB production rate). Activates poorly lecithin:cholesterol acyltransferase (LCAT) and does not enhance efflux of cholesterol from macrophages 2 PublicationsManual assertion based on experiment in:Ref.4

96-Strip-Wells

915 USD

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800