catalog number :
MBS705900
products type :
ELISA Kit
products full name :
Pig Bone Morphogenetic Protein 2, BMP-2 ELISA Kit
products short name :
Bone Morphogenetic Protein 2 (BMP-2)
products name syn :
Pig Bone Morphogenetic Protein 2 (BMP-2) ELISA Kit; Bone Morphogenetic Protein 2 (BMP-2)
other names :
bone morphogenetic protein 2; Bone morphogenetic protein 2; bone morphogenetic protein 2; BMP-2A; bone morphogenetic protein 2A; bone morphogenetic protein 2; Bone morphogenetic protein 2A; BMP-2A
products gene name :
BMP-2
other gene names :
BMP2; BMP2; BDA2; BMP2A; BMP2A; BMP-2; BMP-2A
uniprot entry name :
BMP2_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of pig BMP-2. No significant cross-reactivity or interference between pig BMP-2 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 15.6 pg/ml -1000 pg/ml. Sensitivity: The minimum detectable dose of pig BMP-2 is typically less than 3.9 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for BMP-2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any BMP-2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BMP-2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BMP-2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
AAF21646.1
ncbi mol weight :
44,702 Da
ncbi pathways :
Adipogenesis Pathway (198832); BDNF Signaling Pathway (712093); BMP Signalling And Regulation Pathway (198910); Basal Cell Carcinoma Pathway (83113); Basal Cell Carcinoma Pathway (525); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Elastic Fibre Formation Pathway (730310); Extracellular Matrix Organization Pathway (576262); Heart Development Pathway (198802)
ncbi summary :
The protein encoded by this gene belongs to the transforming growth factor-beta (TGFB) superfamily. The encoded protein acts as a disulfide-linked homodimer and induces bone and cartilage formation. [provided by RefSeq, Jul 2008]
uniprot summary :
Function: Induces cartilage and bone formation.
size4 :
10x96-Strip-Wells