ELISA/assay

Human TAR DNA-binding protein 43, TARDBP/TDP43 ELISA Kit

MBS705899

48-Strip-Wells

510 USD

ELISA Kit

Human TAR DNA-binding protein 43, TARDBP/TDP43 ELISA Kit

TAR DNA binding protein

Human TAR DNA-binding protein 43 (TARDBP/TDP43) ELISA kit; RP4-635E18.2; ALS10; TDP-43; TAR DNA-binding protein-43; TAR DNA binding protein

TAR DNA-binding protein 43; TAR DNA-binding protein 43; TAR DNA-binding protein 43; TAR DNA-binding protein-43; TAR DNA binding protein

TARDBP

TARDBP; TARDBP; ALS10; TDP-43; TDP43; TDP-43

TADBP_HUMAN

Human

414

This assay has high sensitivity and excellent specificity for detection of human TARDBP. No significant cross-reactivity or interference between human TARDBP and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, cell culture supernates, cerebrospinal fluid (CSF). Assay Type: Sandwich. Detection Range: 0.312 ng/ml-20 ng/ml. Sensitivity: Typically less than 0.078 ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TARDBP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TARDBP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TARDBP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TARDBP bound in the initial step. The color development is stopped and the intensity of the color is measured.

6678271

NP_031401.1

NM_007375.3

Q13148

44,740 Da

Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)

HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20. [provided by RefSeq, Jul 2008]

Function: DNA and RNA-binding protein which regulates transcription and splicing. Involved in the regulation of CFTR splicing. It promotes CFTR exon 9 skipping by binding to the UG repeated motifs in the polymorphic region near the 3'-splice site of this exon. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis. May also be involved in microRNA biogenesis, apoptosis and cell division. Can repress HIV-1 transcription by binding to the HIV-1 long terminal repeat. Stabilizes the low molecular weight neurofilament (NFL) mRNA through a direct interaction with the 3' UTR.2 PublicationsManual assertion based on experiment in:Ref.2

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800