catalog number :
MBS705860
products type :
ELISA Kit
products full name :
Human 3-nitrotyrosine, 3-NT ELISA Kit
products short name :
3-nitrotyrosine (3-NT)
products name syn :
Human 3-nitrotyrosine (3-NT) ELISA Kit; 3-nitrotyrosine (3-NT)
specificity :
This assay has high sensitivity and excellent specificity for detection of human 3-NT. No significant cross-reactivity or interference between human 3-NT and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.156 ng/ml -10 ng/ml. Sensitivity: The minimum detectable dose of human 3-NT is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
. Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for 3-NT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any 3-NT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for 3-NT is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of 3-NT bound in the initial step. The color development is stopped and the intensity of the color is measured.
size4 :
10x96-Strip-Wells