ELISA/assay

Human Fibroblast growth factor 21, FGF21/UNQ3115/PRO10196 ELISA Kit

MBS705859

96 Strip Wells

850 USD

ELISA Kit

Human Fibroblast growth factor 21, FGF21/UNQ3115/PRO10196 ELISA Kit

fibroblast growth factor 21

Human Fibroblast growth factor 21 (FGF21/UNQ3115/PRO10196) ELISA kit; fibroblast growth factor 21

fibroblast growth factor 21; Fibroblast growth factor 21; fibroblast growth factor 21; fibroblast growth factor 21

FGF21

FGF21; FGF21; UNQ3115/PRO10196; FGF-21

FGF21_HUMAN

Human

209

This assay has high sensitivity and excellent specificity for detection of human FGF21. No significant cross-reactivity or interference between human FGF21 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: For the quantitative determination of human fibroblast growth factor 21 (FGF21/UNQ3115/PRO10196) concentrations in serum, plasma, cell culture supernates, tissue homogenates. Detection Range: 15.6 pg/ml-1000 pg/ml.

Sensitivity: The minimum detectable dose of human FGF21 is typically less than 3.9 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Priciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FGF21 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FGF21 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FGF21 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FGF21 bound in the initial step. The color development is stopped and the intensity of the color is measured.

9506597

NP_061986.1

NM_019113.2

Q9NSA1

22,300 Da

MAPK Signaling Pathway 83048!!MAPK Signaling Pathway 456!!Melanoma Pathway 83114!!Melanoma Pathway 526!!PI3K-Akt Signaling Pathway 692234!!PI3K-Akt Signaling Pathway 692979!!Pathways In Cancer 83105!!Proteoglycans In Cancer Pathway 782000!!Proteoglycans In Cancer Pathway 782054!!Rap1 Signaling Pathway 868086

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. The function of this growth factor has not yet been determined. [provided by RefSeq, Jul 2008]

Function: Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB.2 PublicationsManual assertion based on experiment in:Ref.7

96 Strip Wells

850 USD