ELISA/assay

Mouse suppressors of cytokine signaling 1, SOCS-1 ELISA Kit

MBS705857

48-Strip-Wells

565 USD

ELISA Kit

Mouse suppressors of cytokine signaling 1, SOCS-1 ELISA Kit

suppressors of cytokine signaling 1 (SOCS-1)

Mouse suppressors of cytokine signaling 1 (SOCS-1) ELISA Kit; CIS1; CISH1; JAB; SOCS-1; SSI-1; SSI1; TIP3; JAK binding protein; STAT induced SH3 protein 1; Tec-interacting protein 3; cytokine-inducible SH2 protein 1; suppressors of cytokine signaling 1 (SOCS-1)

suppressor of cytokine signaling 1; Suppressor of cytokine signaling 1; suppressor of cytokine signaling 1; JAK2-binding protein; STAT-induced STAT inhibitor 1; cytokine inducible SH2-containing protein 1; cytokine inducible SH2-containing protein 7; suppressor of cytokine signaling 1; JAK-binding protein; JAB; STAT-induced STAT inhibitor 1; SSI-1

SOCS-1

Socs1; Socs1; JAB; Cish1; Cish7; SSI-1; SOCS-1; Cish1; Ssi1; SOCS-1; JAB; SSI-1

SOCS1_MOUSE

Mouse

212

This assay has high sensitivity and excellent specificity for detection of mouse SOCS-1. No significant cross-reactivity or interference between mouse SOCS-1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 62.5 pg/ml -4000 pg/ml. Sensitivity: 15.6 pg/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SOCS-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SOCS-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SOCS-1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SOCS-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

409971432

NP_001258532.1

NM_001271603.1

O35716

23,715 Da

Adaptive Immune System Pathway (971210); Adipogenesis Pathway (198299); Antigen Processing: Ubiquitination Proteasome Degradation Pathway (1001197); Class I MHC Mediated Antigen Processing Presentation Pathway (1001196); Cytokine Signaling In Immune System Pathway (970814); ECS Complex Pathway (421852); ECS Complex Pathway (468394); EGFR1 Signaling Pathway (198278); EPO Receptor Signaling Pathway (198317); Growth Hormone Receptor Signaling Pathway (1001294)

Function: SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS1 is involved in negative regulation of cytokines that signal through the JAK/STAT3 pathway. Through binding to JAKs, inhibits their kinase activity. In vitro, also suppresses Tec protein-tyrosine activity Appears to be a major regulator of signaling by interleukin 6 (IL6) and leukemia inhibitory factor (LIF). Regulates interferon-gamma mediated sensory neuron survival. Probable substrate recognition component of an ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize JAK2 SOCS1 appears to be a negative regulator in IGF1R signaling pathway 2 PublicationsManual assertion based on experiment in:Ref.14

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150