ELISA/assay

Human endothelial cell-specific molecule 1, ESM-1 ELISA Kit

MBS705843

48-Strip-Wells

510 USD

ELISA Kit

Human endothelial cell-specific molecule 1, ESM-1 ELISA Kit

endothelial cell-specific molecule 1

Human endothelial cell-specific molecule 1 (ESM-1) ELISA Kit; endocan; ; endothelial cell-specific molecule 1

endothelial cell-specific molecule 1 isoform b; Endothelial cell-specific molecule 1; endothelial cell-specific molecule 1; ESM-1; endothelial cell-specific molecule 1

ESM1

ESM1; ESM1; endocan; ESM-1

ESM1_HUMAN

Human

134

This assay has high sensitivity and excellent specificity for detection of human ESM-1. No significant cross-reactivity or interference between human ESM-1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 0.156 ng/ml -10 ng/ml. Sensitivity: The minimum detectable dose of human ESM-1 is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ESM-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ESM-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ESM-1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ESM-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

208022668

NP_001129076.1

NM_001135604.1

Q9NQ30

20,095 Da

Gastric Cancer Network 1 Pathway (760635)

This gene encodes a secreted protein which is mainly expressed in the endothelial cells in human lung and kidney tissues. The expression of this gene is regulated by cytokines, suggesting that it may play a role in endothelium-dependent pathological disorders. The transcript contains multiple polyadenylation and mRNA instability signals. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008]

Function: Involved in angiogenesis; promotes angiogenic sprouting. May have potent implications in lung endothelial cell-leukocyte interactions.1 PublicationManual assertion based on experiment in:Ref.7

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800