Human Carbonyl reductase [NADPH] 1, CBR1/CBR/CRN ELISA Kit | MyBioSource MBS705832 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Carbonyl reductase [NADPH] 1, CBR1/CBR/CRN ELISA Kit

catalog :

MBS705832

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS705832

products type :

ELISA Kit

products full name :

Human Carbonyl reductase [NADPH] 1, CBR1/CBR/CRN ELISA Kit

products short name :

carbonyl reductase 1

products name syn :

Human Carbonyl reductase [NADPH] 1 (CBR1/CBR/CRN) ELISA kit; CBR; SDR21C1; hCBR1; carbonyl reductase (NADPH) 1; prostaglandin 9-ketoreductase; prostaglandin-E (2) 9-reductase; short chain dehydrogenase/reductase family 21C; member 1; carbonyl reductase 1

other names :

carbonyl reductase; Carbonyl reductase [NADPH] 1; carbonyl reductase [NADPH] 1; carbonyl reductase [NADPH] 1; carbonyl reductase (NADPH) 1; prostaglandin 9-ketoreductase; prostaglandin-E(2) 9-reductase; NADPH-dependent carbonyl reductase 1; 15-hydroxyprostaglandin dehydrogenase; short chain dehydrogenase/reductase family 21C, member 1; carbonyl reductase 1; 15-hydroxyprostaglandin dehydrogenase [NADP(+)] (EC:1.1.1.197); NADPH-dependent carbonyl reductase 1; Prostaglandin 9-ketoreductase; Prostaglandin-E(2) 9-reductase (EC:1.1.1.189)

products gene name :

CBR1

other gene names :

CBR1; CBR1; CBR; hCBR1; SDR21C1; CBR; CRN

uniprot entry name :

CBR1_HUMAN

reactivity :

Human

sequence length :

173

specificity :

This assay has high sensitivity and excellent specificity for detection of human CBR1. No significant cross-reactivity or interference between human CBR1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 31.25 pg/ml -2000 pg/ml. Sensitivity: 7.81 pg/ml.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CBR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CBR1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CBR1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CBR1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

557948119

ncbi acc num :

NP_001273718.1

ncbi gb acc num :

NM_001286789.1

uniprot acc num :

P16152

ncbi mol weight :

30,375 Da

ncbi pathways :

Arachidonic Acid Metabolism Pathway (82991); Arachidonic Acid Metabolism Pathway (685553); Arachidonic Acid Metabolism Pathway (366); Chemical Carcinogenesis Pathway (673221); Chemical Carcinogenesis Pathway (673237); Metabolic Pathways (132956); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); Metabolism Of Xenobiotics By Cytochrome P450 Pathway (83031); Metabolism Of Xenobiotics By Cytochrome P450 Pathway (425)

ncbi summary :

The protein encoded by this gene belongs to the short-chain dehydrogenases/reductases (SDR) family, which function as NADPH-dependent oxidoreductases having wide specificity for carbonyl compounds, such as quinones, prostaglandins, and various xenobiotics. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Nov 2013]

uniprot summary :

Function: NADPH-dependent reductase with broad substrate specificity. Catalyzes the reduction of a wide variety of carbonyl compounds including quinones, prostaglandins, menadione, plus various xenobiotics. Catalyzes the reduction of the antitumor anthracyclines doxorubicin and daunorubicin to the cardiotoxic compounds doxorubicinol and daunorubicinol. Can convert prostaglandin E2 to prostaglandin F2-alpha. Can bind glutathione, which explains its higher affinity for glutathione-conjugated substrates. Catalyzes the reduction of S-nitrosoglutathione.4 PublicationsManual assertion based on experiment in:Ref.14. Catalytic activity: R-CHOH-R' + NADP+ = R-CO-R' + NADPH. Enzyme regulation: Inhibited by quercetin, rutenin and its derivatives.2 PublicationsManual assertion based on experiment in:Ref.14

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

810

size3 :

5x96-Strip-Wells

price3 :

2750

size4 :

10x96-Strip-Wells

price4 :

5150

more info or order :

MyBioSource product webpage